Typical Testing Data/Standard Curve (for reference only)
Rat Activin receptor type-1 (ACVR1) ELISA Kit | ACVR1 elisa kit
Rat Activin receptor type-1 (ACVR1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ACVR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ACVR1 antibody and an ACVR1-HRP conjugate. The assay sample and buffer are incubated together with ACVR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACVR1 concentration since ACVR1 from samples and ACVR1-HRP conjugate compete for the anti-ACVR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACVR1 from the sample, fewer sites are left to bind ACVR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACVR1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. This gene encodes activin A type I receptor which signals a particular transcriptional response in concert with activin type II receptors. Mutations in this gene are associated with fibrodysplasia ossificans progressive. [provided by RefSeq, Jul 2008]
Uniprot Description
ALK2: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin. May be involved for left-right pattern formation during embryogenesis. Interacts with FKBP1A. Interacts with FCHO1. Expressed in normal parenchymal cells, endothelial cells, fibroblasts and tumor-derived epithelial cells. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Protein type: Membrane protein, integral; Kinase, protein; EC 2.7.11.30; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; TKL group; STKR family; Type1 subfamily
Chromosomal Location of Human Ortholog: 2q23-q24
Cellular Component: integral to plasma membrane; apical part of cell; activin receptor complex
Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; activin receptor activity, type I; transforming growth factor beta binding; transforming growth factor beta receptor activity, type I; metal ion binding; peptide hormone binding; activin binding; SMAD binding; ATP binding; transmembrane receptor protein serine/threonine kinase activity; protein kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; protein amino acid phosphorylation; germ cell development; BMP signaling pathway; transforming growth factor beta receptor signaling pathway; neural crest cell migration; pharyngeal system development; in utero embryonic development; peptidyl-threonine phosphorylation; gastrulation with mouth forming second; positive regulation of bone mineralization; negative regulation of signal transduction; patterning of blood vessels; positive regulation of osteoblast differentiation; negative regulation of activin receptor signaling pathway; mesoderm formation; regulation of skeletal muscle development; positive regulation of transcription from RNA polymerase II promoter; smooth muscle cell differentiation; determination of left/right symmetry; regulation of ossification; acute inflammatory response; urogenital system development; G1/S transition of mitotic cell cycle
Disease: Fibrodysplasia Ossificans Progressiva
Research Articles on ACVR1
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Product Notes
The Rat ACVR1 acvr1 (Catalog #AAA7234359) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7234359 ELISA Kit recognizes Rat ACVR1. It is sometimes possible for the material contained within the vial of "Activin receptor type-1 (ACVR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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