Rabbit anti-Human SKA2 Polyclonal Antibody | anti-SKA2 antibody

SKA2 Antibody

Cat. Num. AAA9631302

• Gene Names: SKA2; FAM33A
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SKA2; Polyclonal Antibody; SKA2 Antibody; FAM33A; Family with sequence similarity 33; member A; FLJ12758; MGC110975; Protein FAM33A; SKA 2; SKA2_HUMAN; Spindle and kinetochore associated complex subunit 2; Spindle and kinetochore associated protein 2; Spindle and kinetochore-associated protein 2; Spindle and KT (kinetochore) associated 2; Spindle and KT associated 2; anti-SKA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SKA2 Antibody detects endogenous levels of SKA2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SKA2 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human SKA2.
• Conjugation: Unconjugated
• Similarity: Belongs to the SKA2 family.
• Subunit Structure: Component of the SKA1 complex, composed of SKA1, SKA2 and SKA3. Forms a heterodimer with SKA1; the heterodimer interacting with SKA3. The core SKA1 complex is composed of 2 SKA1-SKA2 heterodimers, each heterodimer interacting with a molecule of the SKA3 homodimer. The core SKA1 complex associates with microtubules and forms oligomeric assemblies. Interacts directly with SKA1. Binds directly to microtubules; but with a much lower affinity than SKA1. May interact with NR3C1; the relevance of such interaction remains unclear in vivo.
• Function: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules.
• Note: Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-SKA2 antibody

Cytoplasm; Cytoskeleton; Spindle; Chromosome; Centromere; Kinetochore; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 32699054
• NCBI GeneID: 348235
• NCBI Accession #: NP_872426
• NCBI GenBank Nucleotide #: NM_182620.3
• UniProt Accession #: Q8WVK7; A6NIL3; B3KPL3; E9PCB8
• Molecular Weight: 8,282 Da
• NCBI Official Full Name: spindle and kinetochore-associated protein 2 isoform 1
• NCBI Official Synonym Full Names: spindle and kinetochore associated complex subunit 2
• NCBI Official Symbol: SKA2
• NCBI Official Synonym Symbols: FAM33A
• NCBI Protein Information: spindle and kinetochore-associated protein 2; spindle and KT (kinetochore) associated 2; family with sequence similarity 33, member A
• UniProt Protein Name: Spindle and kinetochore-associated protein 2
• Protein Family: Spindle and kinetochore-associated protein
• UniProt Gene Name: SKA2
• UniProt Synonym Gene Names: FAM33A
• UniProt Entry Name: SKA2_HUMAN

Uniprot Description

SKA2: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization. Belongs to the SKA2 family.

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: spindle microtubule; outer kinetochore of condensed chromosome; cytosol

Molecular Function: protein binding; microtubule binding

Biological Process: mitosis; cell division; mitotic cell cycle; regulation of microtubule polymerization or depolymerization; chromosome segregation