Rabbit anti-Mouse Sirtuin 1 (SIRT1) Polyclonal Antibody | anti-SIRT1 antibody

Polyclonal Antibody to Sirtuin 1 (SIRT1)

Cat. Num. AAA2002904

• Gene Names: Sirt1; Sir2; Sir2a; SIR2L1; AA673258; Sir2alpha
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Sirtuin 1 (SIRT1); Polyclonal Antibody; Polyclonal Antibody to Sirtuin 1 (SIRT1); anti-SIRT1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against SIRT1. It has been selected for its ability to recognize SIRT1 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with a His-tag and its sequence is listed below.; MGHHHHHHSGSEF-EDAVK LLQECKKIIV LTGAGVSVSC GIPDFRSRDG IYARLAVDFP DLPDPQAMFD IEYFRKDPRP FFKFAKEIYP GQFQPSLCHK FIALSDKEGK LLRNYTQNID TLEQVAGIQR ILQCHGSFAT ASCLICKYKV DCEAVRGDIF NQVVPRCPRC PADEPLAIMK PEIVFFGENL PEQFHRAMKY DKDEVDLLIV IGSSLKVRPV ALIPSSIPHE VPQILINREP LPHLHFDVEL LGDCDVIINE LCHRLGGEYA
• Sequence Length: 420

• Applicable Applications for anti-SIRT1 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant SIRT1 (Glu236~Ala490) expressed in E.coli.
• Cross Reactivity: Human, Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2075768
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Mouse Serum; Lane2: Human Jurkat Cells; Lane3: Human Hela Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant SIRT1, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Lung tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 9790229
• NCBI GeneID: 93759
• NCBI Accession #: NP_062786.1
• NCBI GenBank Nucleotide #: NM_019812.3
• UniProt Accession #: Q923E4; Q9QXG8
• Molecular Weight: 59,875 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-1 isoform 1
• NCBI Official Synonym Full Names: sirtuin 1
• NCBI Official Symbol: Sirt1
• NCBI Official Synonym Symbols: Sir2; Sir2a; SIR2L1; AA673258; Sir2alpha
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-1
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-1
• Protein Family: NAD-dependent protein deacetylase sirtuin
• UniProt Gene Name: Sirt1
• UniProt Synonym Gene Names: Sir2l1; Sir2; mSIR2a; 75SirT1

NCBI Description

This gene encodes a member of the sirtuin family of proteins, characterized by their deacetylase activity and proposed role in longevity. The encoded protein regulates gene expression in a wide range of cell and tissue types through its NAD+-dependent deacetylation of histones, transcription factors and transcriptional coactivators. Brain-specific overexpression of this gene has been shown to result in increased median lifespan. Viability of homozygous knockout mice for this gene varies with strain background. Homozygous knockout mice of strains that do not exhibit embryonic lethality are sterile and have a reduced lifespan. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

SIRT1: an NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separate cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. Elevation of NAD(+)/NADP(+) ratio activates SIRT1. Recruited to LRH1 target gene promoters by NR0B2/SHP thereby stimulating histone H3 and H4 deacetylation leading to transcriptional repression. Implicated in regulation of adipogenesis and fat mobilization in white adipocytes by repression of PPARG. Involved in liver and muscle metabolism. Is involved in autophagy, presumably by deacetylating ATG5, ATG7 and ATG8. Deacetylates AKT1 which leads to enhanced binding of AKT1 and PDK1 to PIP3 and promotes their activation. Widely expressed. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Deacetylase; EC 3.5.1.-; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 10|10 B4

Cellular Component: axon; chromatin; chromatin silencing complex; cytoplasm; cytosol; ESC/E(Z) complex; growth cone; mitochondrion; nuclear chromatin; nuclear envelope; nuclear euchromatin; nuclear heterochromatin; nuclear inner membrane; nucleoplasm; nucleus; PML body

Molecular Function: bHLH transcription factor binding; deacetylase activity; enzyme binding; histone binding; histone deacetylase activity; HLH domain binding; hydrolase activity; identical protein binding; keratin filament binding; metal ion binding; mitogen-activated protein kinase binding; NAD+ binding; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); nuclear hormone receptor binding; p53 binding; protein binding; protein C-terminus binding; protein deacetylase activity; protein domain specific binding; protein kinase B binding; transcription corepressor activity; transcription factor binding

Biological Process: angiogenesis; apoptosis; behavioral response to starvation; cell differentiation; cell glucose homeostasis; cellular response to DNA damage stimulus; cellular response to hydrogen peroxide; cellular response to hypoxia; cellular response to ionizing radiation; cellular response to starvation; cellular response to tumor necrosis factor; cholesterol homeostasis; chromatin organization; chromatin silencing at rDNA; circadian regulation of gene expression; circadian rhythm; DNA damage response, signal transduction resulting in induction of apoptosis; DNA synthesis involved in DNA repair; establishment of chromatin silencing; fatty acid homeostasis; histone deacetylation; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; leptin-mediated signaling pathway; macrophage cytokine production; macrophage differentiation; maintenance of chromatin silencing; multicellular organism development; muscle organ development; negative regulation of apoptosis; negative regulation of cAMP-dependent protein kinase activity; negative regulation of cardiac muscle cell apoptotic process; negative regulation of cell growth; negative regulation of cellular response to testosterone stimulus; negative regulation of cellular senescence; negative regulation of DNA binding; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of fat cell differentiation; negative regulation of fibroblast apoptotic process; negative regulation of gene expression; negative regulation of growth hormone secretion; negative regulation of helicase activity; negative regulation of histone H3-K14 acetylation; negative regulation of histone H3-K9 trimethylation; negative regulation of histone H4-K16 acetylation; negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; negative regulation of neuron apoptosis; negative regulation of neuron death; negative regulation of NF-kappaB transcription factor activity; negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway; negative regulation of peptidyl-lysine acetylation; negative regulation of phosphorylation; negative regulation of prostaglandin biosynthetic process; negative regulation of protein acetylation; negative regulation of protein kinase B signaling cascade; negative regulation of protein localization to nucleus; negative regulation of TOR signaling; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of tumor necrosis factor production; ovulation from ovarian follicle; peptidyl-lysine acetylation; positive regulation of adaptive immune response; positive regulation of adipose tissue development; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of blood vessel diameter; positive regulation of cAMP-dependent protein kinase activity; positive regulation of cell proliferation; positive regulation of cellular senescence; positive regulation of cholesterol efflux; positive regulation of chromatin silencing; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of DNA repair; positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway; positive regulation of endothelial cell proliferation; positive regulation of gluconeogenesis; positive regulation of heart rate; positive regulation of histone H3-K9 methylation; positive regulation of insulin receptor signaling pathway; positive regulation of macroautophagy; positive regulation of macrophage apoptotic process; positive regulation of MHC class II biosynthetic process; positive regulation of neuron projection development; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein deacetylation; positive regulation of protein phosphorylation; positive regulation of skeletal muscle cell proliferation; positive regulation of smooth muscle cell differentiation; positive regulation of thyroid-stimulating hormone secretion; positive regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; protein deacetylation; protein destabilization; protein ubiquitination; pyrimidine dimer repair via nucleotide-excision repair; regulation of apoptosis; regulation of brown fat cell differentiation; regulation of cell proliferation; regulation of endodeoxyribonuclease activity; regulation of energy homeostasis; regulation of glucose metabolic process; regulation of lipid storage; regulation of mitotic cell cycle; regulation of protein import into nucleus, translocation; regulation of protein serine/threonine kinase activity; regulation of smooth muscle cell apoptotic process; regulation of transcription, DNA-templated; response to ethanol; response to hydrogen peroxide; response to insulin stimulus; response to leptin; response to oxidative stress; rhythmic process; rRNA processing; single strand break repair; spermatogenesis; stress-induced premature senescence; transcription, DNA-dependent; triacylglycerol mobilization; white fat cell differentiation