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Immunohistochemistry (IHC) (Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded convoluted tubules of normal rat kidney tissue (Fig 1 and 2) was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of Sirt5. Reactivity was detected using the IHC-Select Detection Kit. Positive granular cutoplasmic staining was observed in convoluted tubules of normal rat kidney tissue.)

Rabbit anti-Human, Rat SIRT5 Polyclonal Antibody | anti-SIRT5 antibody

SIRT5 (NAD-dependent Deacetylase Sirtuin 5, SIR2-like Protein 5, SIR2L5) (HRP)

Gene Names
SIRT5; SIR2L5
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
SIRT5; Polyclonal Antibody; SIRT5 (NAD-dependent Deacetylase Sirtuin 5; SIR2-like Protein 5; SIR2L5) (HRP); anti-SIRT5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SIRT5. Species Crossreactivity: rat.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-SIRT5 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:500
WB: 0.5ug/ml
Applications are based on unconjugated antibody.
Immunogen
Synthetic linear peptide corresponding to human SIRT5 (KLH). Species sequence homology: chimpanzee and rhesus macaque (100%).
Conjugate
HRP
Positive Control
HeLa Cell Lysate
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Immunohistochemistry (IHC)

(Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded convoluted tubules of normal rat kidney tissue (Fig 1 and 2) was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of Sirt5. Reactivity was detected using the IHC-Select Detection Kit. Positive granular cutoplasmic staining was observed in convoluted tubules of normal rat kidney tissue.)

Immunohistochemistry (IHC) (Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded convoluted tubules of normal rat kidney tissue (Fig 1 and 2) was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of Sirt5. Reactivity was detected using the IHC-Select Detection Kit. Positive granular cutoplasmic staining was observed in convoluted tubules of normal rat kidney tissue.)

Western Blot (WB)

(Western Blotting Analysis: Representative lot data. HeLa cell lysate was probed with Sirt5 (0.5ug/ml). Proteins were visualized using a Donkey Rabbit IgG secondary antibodies conjugated to HRP and a chemiluminescence detection system. Arrow indicates Sirt5 (~34kD).)

Western Blot (WB) (Western Blotting Analysis: Representative lot data. HeLa cell lysate was probed with Sirt5 (0.5ug/ml). Proteins were visualized using a Donkey Rabbit IgG secondary antibodies conjugated to HRP and a chemiluminescence detection system. Arrow indicates Sirt5 (~34kD).)
Product Categories/Family for anti-SIRT5 antibody
References
1. Matsushita, N., et al. (2011). Genes Cells. 16(2):190-202. 2. Nakagawa, T. and Guarente, L. (2009). Aging (Albany NY). 1(6):578-581. 3. Mahlknecht, U., et al. (2006). Cytogenet Genome Res. 112(3-4):208-212.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,689 Da
NCBI Official Full Name
NAD-dependent protein deacylase sirtuin-5, mitochondrial isoform 1
NCBI Official Synonym Full Names
sirtuin 5
NCBI Official Symbol
SIRT5
NCBI Official Synonym Symbols
SIR2L5
NCBI Protein Information
NAD-dependent protein deacylase sirtuin-5, mitochondrial; SIRT5; sir2-like 5; sirtuin type 5; NAD-dependent deacetylase sirtuin-5; silent mating type information regulation 2, S.cerevisiae, homolog 5; NAD-dependent lysine demalonylase and desuccinylase si
UniProt Protein Name
NAD-dependent protein deacylase sirtuin-5, mitochondrial
UniProt Gene Name
SIRT5
UniProt Synonym Gene Names
SIR2L5
UniProt Entry Name
SIR5_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2010]

Research Articles on SIRT5

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Product Notes

The SIRT5 sirt5 (Catalog #AAA6270115) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIRT5 (NAD-dependent Deacetylase Sirtuin 5, SIR2-like Protein 5, SIR2L5) (HRP) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SIRT5 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:500 WB: 0.5ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SIRT5 sirt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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