Goat anti-Human SIRT3 Polyclonal Antibody | anti-Sirt3 antibody

SIRT3 (Sirtuin 3, Sirtuin-3, Sirtuin Silent Mating Type Information Regulation 2 Homolog 3 (S. cerevisiae), Sirtuin Type 3, HGNC:14931, hSIRT3, Mitochondrial Nicotinamide Adenine Dinucleotide-dependent Deacetylase, NAD-dependent Deacetylase Sirtuin 3 Mito

Cat. Num. AAA631266

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by peptide affinity chromatography.
• Synonyms: SIRT3; Polyclonal Antibody; SIRT3 (Sirtuin 3; Sirtuin-3; Sirtuin Silent Mating Type Information Regulation 2 Homolog 3 (S. cerevisiae); Sirtuin Type 3; HGNC:14931; hSIRT3; Mitochondrial Nicotinamide Adenine Dinucleotide-dependent Deacetylase; NAD-dependent Deacetylase Sirtuin 3 Mito; Anti -SIRT3 (Sirtuin 3; anti-Sirt3 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SIRT3. This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).
• Purity/Purification: Affinity Purified; Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-Sirt3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Peptide ELISA Titer: 1:128,000; Western Blot: 0.1-0.3ug/ml ~28kD band observed in Human Liver lysates (calculated MW of 28.6kD according to NP_001017524.1). In transfected HEK293 transiently expressing SIRT3 bands of approx. 35kD and 48kD are observed. These bands are not observed in the non-transfected HEK293.
• Immunogen: Synthetic peptide corresponding to C-QRETGKLDGPDK, from the C Terminus of human SIRT3(NP_036371.1, NP_001017524.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(S1013-88F4 (0.2µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Testing Data

(HEK293 overexpressing SIRT3 and probed with S1013-88F4 (mock transfection in first lane), tested by Origene)

Related Product Information for anti-Sirt3 antibody

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.

Product Categories/Family for anti-Sirt3 antibody

Antibodies; Abs to Sirtuins

NCBI and Uniprot Product Information

• NCBI GI #: 71060129
• UniProt Accession #: Q4FJK3
• Molecular Weight: 28,822 Da
• NCBI Official Full Name: Sirt3
• UniProt Protein Name: Sirt3 protein
• Protein Family: NAD-dependent protein deacetylase sirtuin
• UniProt Gene Name: Sirt3
• UniProt Entry Name: Q4FJK3_MOUSE

Uniprot Description

SIRT3: a soluble mitochondrial NAD-dependent protein deacetylase. Can deacetylate and thereby activate a central metabolic regulator in the mitochondrial matrix, glutamate dehydrogenase. Mitochondrial proteins are hyperacetylated in SIRT3-deficient mice, but not in SIRT4 or SIRT5 deficient mice. SIRT3-deficient mice have no obvious metabolic phenotype, and the process of adaptive thermogenesis is normal. Can deacetylate and activate isocitrate dehydrogenase 2, an enzyme that participates in regulation of the citric acid cycle and the regeneration of antioxidants. Inhibited by nicotinamide, but not by trichostatin A. Its unprocessed form is enzymatically inactive. It is processed by mitochondrial processing peptidase (MPP) in the mitochondrial matrix to give an enzymatically active 28 kDa product.

Protein type: Mitochondrial; EC 3.5.1.-; Deacetylase

Cellular Component: membrane; mitochondrion; mitochondrial matrix; cytoplasm

Molecular Function: (3,5-dichlorophenylurea)acetate amidohydrolase activity; hydrolase activity; zinc ion binding; metal ion binding; UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; N-acetylgalactosamine-6-phosphate deacetylase activity; N2-acetyl-L-lysine deacetylase activity; iprodione amidohydrolase activity; protein binding; enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); indoleacetamide hydrolase activity; protein N-terminal asparagine amidohydrolase activity; histone deacetylase activity; O-succinylbenzoate synthase activity; NAD binding

Biological Process: protein amino acid deacetylation; aerobic respiration; positive regulation of insulin secretion