Rabbit anti-Human SIRT3 Polyclonal Antibody | anti-SIRT3 antibody

SIRT3, NT (SIRT3, SIR2L3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, Regulatory protein SIR2 homolog 3, SIR2-like protein 3) (FITC)

Cat. Num. AAA6347805

• Gene Names: SIRT3; SIR2L3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SIRT3; Polyclonal Antibody; NT (SIRT3; SIR2L3; NAD-dependent protein deacetylase sirtuin-3; mitochondrial; Regulatory protein SIR2 homolog 3; SIR2-like protein 3) (FITC); SIR2-like protein 3; anti-SIRT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SIRT3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SIRT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the N-terminal region of human SIRT3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SIRT3 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the SIRT3 antibody detected the SIRT3 protein (arrow).)

Related Product Information for anti-SIRT3 antibody

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.

Product Categories/Family for anti-SIRT3 antibody

Antibodies; Mitochondrial Proteins

References

Dransfeld, C.L., et al. Int. J. Oncol. 36(4):955-960(2010). Shulga, N., et al. J. Cell. Sci. 123 (PT 6), 894-902 (2010): Shi, T., et al. J Dig Dis 11(1):55-62(2010). Kong, X., et al. PLoS ONE 5 (7), E11707 (2010): Li, S., et al. PLoS ONE 5 (5), E10486 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 6912660
• NCBI GeneID: 23410
• NCBI Accession #: NP_036371.1; NP_001017524.1
• NCBI GenBank Nucleotide #: NM_012239.5
• UniProt Accession #: Q9NTG7; Q9Y6E8; B7Z5U6
• Molecular Weight: 28,567 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform a
• NCBI Official Synonym Full Names: sirtuin 3
• NCBI Official Symbol: SIRT3
• NCBI Official Synonym Symbols: SIR2L3
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-3, mitochondrial
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-3, mitochondrial
• Protein Family: NAD-dependent protein deacetylase sirtuin
• UniProt Gene Name: SIRT3
• UniProt Synonym Gene Names: SIR2L3; hSIRT3
• UniProt Entry Name: SIR3_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SIRT3: a soluble mitochondrial NAD-dependent protein deacetylase. Can deacetylate and thereby activate a central metabolic regulator in the mitochondrial matrix, glutamate dehydrogenase. Mitochondrial proteins are hyperacetylated in SIRT3-deficient mice, but not in SIRT4 or SIRT5 deficient mice. SIRT3-deficient mice have no obvious metabolic phenotype, and the process of adaptive thermogenesis is normal. Can deacetylate and activate isocitrate dehydrogenase 2, an enzyme that participates in regulation of the citric acid cycle and the regeneration of antioxidants. Inhibited by nicotinamide, but not by trichostatin A. Its unprocessed form is enzymatically inactive. It is processed by mitochondrial processing peptidase (MPP) in the mitochondrial matrix to give an enzymatically active 28 kDa product.

Protein type: Deacetylase; EC 3.5.1.-; Mitochondrial

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: mitochondrion; membrane; mitochondrial matrix

Molecular Function: protein binding; enzyme binding; zinc ion binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NAD+ ADP-ribosyltransferase activity

Biological Process: mitochondrion organization and biogenesis; protein amino acid ADP-ribosylation; protein amino acid deacetylation; organelle organization and biogenesis; aerobic respiration; aging