Western Blot (WB)
(Sample Data: Western blot analysis of SIRT1 in mouse liver tissue lysate with SIRT1 antibody at 1 ug/ml.)
Rabbit SIRT1 Polyclonal Antibody | anti-SIRT1 antibody
Anti-SIRT1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Affinity chromatography
Synonyms
SIRT1; Polyclonal Antibody; Anti-SIRT1; anti-SIRT1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the SIRT1 protein
Purity/Purification
Affinity chromatography
Form/Format
PBS + 0.02% sodium azide
Applicable Applications for anti-SIRT1 antibody
Western Blot (WB), ELISA (EIA), Immunofluorescence (IF)
Immunogen
SIRT1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human SIRT1
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of SIRT1 in mouse liver tissue lysate with SIRT1 antibody at 1 ug/ml.)
Western Blot (WB)
(Sample Data: Western blot analysis of SIRT1 in mouse liver tissue lysate with SIRT1 antibody at 1 ug/ml.)
Immunofluorescence (IF)
(Sample Data: Immunofluorescence of SIRT1 in rat lung tissue with SIRT1 antibody at 20 ug/ml.)
Immunofluorescence (IF)
(Sample Data: Immunofluorescence of SIRT1 in rat lung tissue with SIRT1 antibody at 20 ug/ml.)
Related Product Information for anti-SIRT1 antibody
SIRT1 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Each member of the Sirtuin family contain sirtuin core domains. These proteins are grouped into four classes with SIRT1 being a member of class I. SIRT1 is a stress-response and chromatin-silencing factor (1). It is an NAD(+)-dependent histone deacetylase involved in various nuclear events such as transcription, DNA replication, and DNA repair. SIRT1 protein binds and deacetylates the p53 protein (2). Expression of wild type SIRT1 in human cells reduces the transcriptional activity of p53 indicating that SIRT1 is involved in the regulation of p53 function via deacetylation.
Product Categories/Family for anti-SIRT1 antibody
References
1. Tanny, J. C. et al: An enzymatic activity in the yeast Sir2 protein that is essential for gene silencing. Cell 99: 735-745, 1999.
2. Vaziri, H. et al: hSIR2-SIRT1 functions as an NAD-dependent p53 deacetylase. Cell 107: 149-159, 2001.
2. Vaziri, H. et al: hSIR2-SIRT1 functions as an NAD-dependent p53 deacetylase. Cell 107: 149-159, 2001.
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Product Notes
The SIRT1 (Catalog #AAA516225) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SIRT1 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SIRT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF). Researchers should empirically determine the suitability of the SIRT1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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