Western Blot (WB)
(Sample Data: Western blot analysis of SIRT3 in mouse heart tissue lysate with SIRT3 antibody at (A) 1 and (B) 2 ug/ml.)
Rabbit SIRT3 Polyclonal Antibody | anti-SIRT3 antibody
Anti-SIRT3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity chromatography
Synonyms
SIRT3; Polyclonal Antibody; Anti-SIRT3; anti-SIRT3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the SIRT3 protein
Purity/Purification
Affinity chromatography
Form/Format
PBS + 0.02% sodium azide
Applicable Applications for anti-SIRT3 antibody
Western Blot (WB), ELISA
Immunogen
SIRT3 antibody was raised against a 17 amino acid synthetic peptide near the center of human SIRT3
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of SIRT3 in mouse heart tissue lysate with SIRT3 antibody at (A) 1 and (B) 2 ug/ml.)
Western Blot (WB)
(Sample Data: Western blot analysis of SIRT3 in mouse heart tissue lysate with SIRT3 antibody at (A) 1 and (B) 2 ug/ml.)
Related Product Information for anti-SIRT3 antibody
SIRT3 is a member of the class I of the Sirtuin family that is characterized by a Sirtuin core domain. Sirtuin are grouped into four classes and are homologs to the Sir2 protein. SIRT3 is known to regulate the epigenetic gene silencing and suppresses recombination of rDNA (1). SIRT3 may function as an intracellular regulatory protein with mono-ADP-ribosyltransferase activity that is essential for its silencing function. SIRT3 regulates and maintains basal ATP levels in the cell (2). SIRT3 also modulates mitochondrial intermediary metabolism and fatty acid use during fasting (3).
Product Categories/Family for anti-SIRT3 antibody
References
1. Tanny, J. C. et.al: An enzymatic activity in the yeast Sir2 protein that is essential for gene silencing. Cell 99: 735-745, 1999.
2. Ahn, B.-H. et.al: A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Proc. Nat. Acad. Sci. 105: 14447-14452, 2008.
3. Hirschey, M. D. et.al: SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation. Nature 464: 121-125, 2010.
2. Ahn, B.-H. et.al: A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Proc. Nat. Acad. Sci. 105: 14447-14452, 2008.
3. Hirschey, M. D. et.al: SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation. Nature 464: 121-125, 2010.
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Product Notes
The SIRT3 (Catalog #AAA516229) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SIRT3 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SIRT3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA. Researchers should empirically determine the suitability of the SIRT3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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