Western Blot (WB)
(Western blot analysis of extracts from MOLT cells using Lck (phospho-Tyr393) antibody)
Rabbit LCK Polyclonal Antibody | anti-LCK antibody
LCK Antibody (Tyr393)
Gene Names
LCK; LSK; YT16; IMD22; p56lck; pp58lck
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
LCK; Polyclonal Antibody; LCK Antibody (Tyr393); Rabbit Polyclonal LCK Antibody (Tyr393); Polyclonal Lck antibody; Anti-Lck antibody; LCK antibody; LSK antibody; Lymphocyte cell-specific protein-tyrosine kinase antibody; lymphocyte-specific protein tyrosine kinase antibody; p56(LSTRA) protein-tyrosine kinase antibody; p56-LCK antibody; p56lck antibody; pp58lck antibody; Proto-oncogene Lck antibody; proto-oncogene tyrosine-protein kinase LCK antibody; T cell-specific protein-tyrosine kinase antibody; T-lymphocyte specific protein tyrosine kinase p56lck antibody; Tyrosine-protein kinase Lck antibody; YT16 antibody; anti-LCK antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Lck antibody (Tyr393) detects endogenous levels of Lck only when phosphorylated at tyrosine 393
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
35
Applicable Applications for anti-LCK antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Lck antibody was raised in Rabbit using a synthesized phosphopeptide derived from human Lck around the phosphorylation site of tyrosine 393 (N-E-YP-T-A) as the immunogen.
Cross-Reactivity
Mouse, Rat
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts from MOLT cells using Lck (phospho-Tyr393) antibody)
Western Blot (WB)
(Western blot analysis of extracts from MOLT cells using Lck (phospho-Tyr393) antibody)
Related Product Information for anti-LCK antibody
Lck is most commonly found in T cells. It associates with the cytoplasmic tails of the CD4 and CD8 co-receptors on T helper cells and cytotoxic T cells, respectively, to assist signaling from the T cell receptor (TCR) complex. When the T cell receptor is engaged by the specific antigen presented by MHC, Lck acts to phosphorylate the intracellular chains of the CD3 and zeta-chains of the TCR complex, allowing another cytoplasmic tyrosine kinase called ZAP-70 to bind to them.
Product Categories/Family for anti-LCK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
LCK, partial
NCBI Official Synonym Full Names
LCK proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol
LCK
NCBI Official Synonym Symbols
LSK; YT16; IMD22; p56lck; pp58lck
NCBI Protein Information
tyrosine-protein kinase Lck
Protein Family
NCBI Description
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Aug 2016]
Research Articles on LCK
Similar Products
Product Notes
The LCK (Catalog #AAA5313584) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's LCK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the LCK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LCK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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