TrkA Active Protein | NTRK1 active protein

TrkA Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8123129

• Gene Names: Ntrk1; Tkr; trk; TrkA; C80751
• Purity: >90% as determined by SDS-PAGE
• Synonyms: TrkA; TrkA Protein; Mouse; Recombinant (hFc Tag); Mouse TrkA/NTRK1 Protein (Fc Tag); C80751 Protein; Tkr Protein; trk Protein; neurotrophic tyrosine kinase; receptor; type 1; NTRK1 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly420

• Species: Mouse
• Activity: Measured by its ability to inhibit NGF-induced proliferation of TF?1 human erythroleukemic cells. The ED50 for this effect is typically 0.02-0.08ug/mL in the presence of 10ng/mL of recombinant mouse NGF.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 34
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse NTRK1 (NP_001028296.1) (Met1-Gly420) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.02-0.08 ug/mL in the presence of 10 ng/mL of recombinant mouse NGF.)

Related Product Information for NTRK1 active protein

Background: TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by the pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in the TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation, and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as the establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma.

Product Categories/Family for NTRK1 active protein

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

References

Lambiase A, et al. (2005) Molecular basis for keratoconus: lack of TrkA expression and its transcriptional repression by Sp3. Natl Acad Sci. 102 (46):16795-800.
Benito-Gutia©rrez E, et al. (2006) Origin and evolution of the Trk family of neurotrophic receptors. Mol Cell Neurosci. 31(2):179-92.
Martin-Zanca D, et al. (1986) A human oncogene formed by the fusion of truncated tropomyosin and protein tyrosine kinase sequences. Nature. 319(6056):743-8.

NCBI and Uniprot Product Information

• NCBI GI #: 169234626
• NCBI GeneID: 18211
• NCBI Accession #: NP_001028296.1
• NCBI GenBank Nucleotide #: NM_001033124.1
• UniProt Accession #: Q3UFB7
• Molecular Weight: 87,738 Da
• NCBI Official Full Name: high affinity nerve growth factor receptor
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 1
• NCBI Official Symbol: Ntrk1
• NCBI Official Synonym Symbols: Tkr; trk; TrkA; C80751
• NCBI Protein Information: high affinity nerve growth factor receptor
• UniProt Protein Name: High affinity nerve growth factor receptor
• Protein Family: High affinity nerve growth factor receptor
• UniProt Gene Name: Ntrk1
• UniProt Entry Name: NTRK1_MOUSE

Uniprot Description

TrkA: a receptor tyrosine kinase of the Trk family. High affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor. Known substrates include Shc, PI-3K, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system. Two splice variant isoforms have been described.

Protein type: Protein kinase, TK; Membrane protein, integral; Oncoprotein; Protein kinase, tyrosine (receptor); Kinase, protein; EC 2.7.10.1; TK group; Trk family

Cellular Component: protein complex; cell surface; integral to plasma membrane; early endosome; dendrite; integral to membrane; cell soma; membrane; axon; cytoplasm; late endosome; plasma membrane; cytoplasmic vesicle; receptor complex; endosome

Molecular Function: protein homodimerization activity; ephrin receptor binding; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; neurotrophin receptor activity; protein kinase activity; neurotrophin p75 receptor binding; transferase activity; protein binding; nerve growth factor receptor activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nerve growth factor binding; kinase activity; kinase binding; ATP binding

Biological Process: response to nicotine; axon guidance; mechanoreceptor differentiation; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; multicellular organismal development; protein amino acid autophosphorylation; sensory perception of pain; positive regulation of synaptic transmission, glutamatergic; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; sympathetic nervous system development; learning and/or memory; negative regulation of neuron apoptosis; detection of temperature stimulus involved in sensory perception of pain; response to electrical stimulus; cell differentiation; response to nutrient levels; response to drug; nervous system development; Sertoli cell development; detection of mechanical stimulus involved in sensory perception of pain; positive regulation of angiogenesis; positive regulation of programmed cell death; B cell differentiation; response to activity; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway