Rabbit anti-Human CSNK1D Polyclonal Antibody | anti-CSNK1D antibody

CSNK1D, CT (Casein Kinase I, delta Isoform, CKI-delta, CKId) (Biotin)

Cat. Num. AAA6467363

• Gene Names: CSNK1D; ASPS; HCKID; FASPS2; CKIdelta
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CSNK1D; Polyclonal Antibody; CT (Casein Kinase I; delta Isoform; CKI-delta; CKId) (Biotin); anti-CSNK1D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CK1d.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CSNK1D antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CK1d (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CK1d C-term Pab in HeLa cell lysate. CK1d (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-CSNK1D antibody

Antibodies; Protein Kinases

References

1. Sillibourne, J.E., et al., J. Mol. Biol. 322(4):785-797 (2002). 2. Rivers, A., et al., J. Biol. Chem. 273(26):15980-15984 (1998). 3. Kusuda, J., et al., Genomics 32(1):140-143 (1996). 4. Fish, K.J., et al., J. Biol. Chem. 270(25):14875-14883 (1995). 5. Graves, P.R., et al., J. Biol. Chem. 270(37):21689-21694 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 20149530
• NCBI GeneID: 1453
• NCBI Accession #: NP_001884.2
• NCBI GenBank Nucleotide #: NM_001893.4
• UniProt Accession #: P48730; Q96KZ6; Q9BTN5; A2I2P2
• Molecular Weight: 47,330 Da
• NCBI Official Full Name: casein kinase I isoform delta isoform 1
• NCBI Official Synonym Full Names: casein kinase 1, delta
• NCBI Official Symbol: CSNK1D
• NCBI Official Synonym Symbols: ASPS; HCKID; FASPS2; CKIdelta
• NCBI Protein Information: casein kinase I isoform delta; CKId; CKI-delta; tau-protein kinase CSNK1D
• UniProt Protein Name: Casein kinase I isoform delta
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK1D
• UniProt Synonym Gene Names: HCKID; CKI-delta; CKId
• UniProt Entry Name: KC1D_HUMAN

NCBI Description

This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CK1D: an ubiquitous protein kinase of the CK1 family. Enriched at the centrosomes in interphase and at the spindle during mitosis. May play a role in cell cycle progression. Two splice variant isoforms have been described.

Protein type: EC 2.7.11.26; Protein kinase, CK1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; CK1 group; CK1 family

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: Golgi apparatus; centrosome; spindle microtubule; perinuclear region of cytoplasm; plasma membrane; spindle; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; tau-protein kinase activity; phosphoprotein binding; glycoprotein binding; peptide binding; ATP binding; protein kinase activity

Biological Process: Wnt receptor signaling pathway; organelle organization and biogenesis; endocytosis; spindle assembly; signal transduction; regulation of circadian rhythm; DNA repair; protein amino acid phosphorylation; regulation of cell shape; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; mitotic cell cycle; circadian regulation of gene expression; G2/M transition of mitotic cell cycle

Disease: Advanced Sleep Phase Syndrome, Familial, 2