Rabbit anti-Human, mouse SIGLEC5 Polyclonal Antibody | anti-SIGLEC5 antibody

SIGLEC5 Antibody (C-term)

Cat. Num. AAA9210322

• Gene Names: SIGLEC5; CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: SIGLEC5; Polyclonal Antibody; SIGLEC5 Antibody (C-term); Sialic acid-binding Ig-like lectin 5; Siglec-5; CD33 antigen-like 2; Obesity-binding protein 2; OB-BP2; OB-binding protein 2; CD170; CD33L2; OBBP2; anti-SIGLEC5 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SIGLEC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-513 amino acids from the C-terminal region of human SIGLEC5.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 482-513
• Sequence Length: 551

• Applicable Applications for anti-SIGLEC5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(The anti-Siglec5 C-term Pab is used in Western blot to detect Siglec5 in mouse liver tissue lysate.)

Western Blot (WB)

(SIGLEC5 Antibody western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the SIGLEC5 antibody detected the SIGLEC5 protein (arrow).)

Related Product Information for anti-SIGLEC5 antibody

SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have one or more cytoplasmic immune receptor tyrosine-based inhibitory motifs (ITIM). SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. Sequence analysis predicted that the 697-amino acid SIGLEC10 protein contains a signal peptide, an N-terminal V-set Ig-like domain and four C2-set Ig-like domains, five potential N-linked glycosylation sites, a transmembrane region, and a 126-residue cytoplasmic tail with 3 putative ITIMs. Northern blot analysis detected a major 3.0-kb SIGLEC10 transcript, with highest levels in spleen, lymph node, blood leukocytes, and appendix. Little or no expression was observed in pancreas, thyroid, and testis. Flow cytometric analysis demonstrated eosinophil-specific expression of SIGLEC10, but at a lower level than that of SIGLEC8. Expression was also detected on monocytes and a CD16-positive/CD56-negative natural killer-like lymphocyte population. After sialidase treatment, which is necessary for unmasking the sialic acid-binding site on SIGLECs interacting with cell surface sialic acids, cells expressing SIGLEC10 bound to red blood cells. Immunoprecipitation analysis indicated expression of a 100- to 120-kD monomeric protein, higher than the predicted molecular mass, suggesting that SIGLEC10 is glycosylated.

Product Categories/Family for anti-SIGLEC5 antibody

Immunology

References

Erickson-Miller, C.L., et al., Exp. Hematol. 31(5):382-388 (2003).
Patel, N., et al., J. Biol. Chem. 274(32):22729-22738 (1999).
Cornish, A.L., et al., Blood 92(6):2123-2132 (1998).
Kim, H.S., Cytogenet. Cell Genet. 84 (1-2), 96 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4502659
• NCBI GeneID: 8778
• NCBI Accession #: NP_003821.1
• NCBI GenBank Nucleotide #: NM_003830.3
• UniProt Accession #: O15389
• Molecular Weight: 60715
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 5
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin 5
• NCBI Official Symbol: SIGLEC5
• NCBI Official Synonym Symbols: CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• NCBI Protein Information: sialic acid-binding Ig-like lectin 5
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 5
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC5
• UniProt Synonym Gene Names: CD33L2; OBBP2; Siglec-5; OB-BP2; OB-binding protein 2
• UniProt Entry Name: SIGL5_HUMAN

NCBI Description

This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012]

Uniprot Description

SIGLEC5: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to membrane

Molecular Function: carbohydrate binding

Biological Process: cell adhesion