Rabbit SIAH Interacting Protein Polyclonal Antibody | anti-CACYBP antibody

Anti-SIAH Interacting Protein antibody

Cat. Num. AAA175645

• Gene Names: CACYBP; SIP; GIG5; PNAS-107; S100A6BP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: SIAH Interacting Protein; Polyclonal Antibody; Anti-SIAH Interacting Protein antibody; Calcyclin-binding protein(CacyBP/hCacyBP); calcyclin binding protein; CACYBP antibody; Calcyclin binding protein antibody; GIG 5 antibody; GIG5 antibody; Growth inhibiting gene 5 antibody; Growth inhibiting gene 5 protein antibody; hCacyBP antibody; MGC87971 antibody; PNAS 107 antibody; PNAS107 antibody; RP1 102G20.6 antibody; S100A6 binding protein antibody; S100A6BP antibody; SIP antibody; anti-CACYBP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 228

• Applicable Applications for anti-CACYBP antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SIAH Interacting Protein (160-175aa NTRWDYLTQVEKECKE), identical to the related rat and mouse sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-CACYBP antibody, MBS175645, Western blottingAll lanes: Anti CACYBP (MBS175645) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: Rat Spleen Tissue Lysate at 50ugLane 4: SMMC Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: SW620 Whole Cell Lysate at 40ugLane 7: 293T Whole Cell Lysate at 40ugPredicted bind size: 26KDObserved bind size: 26KD)

Immunocytochemistry (ICC)

(Anti-CACYBP antibody, MBS175645, ICCICC: A549 Cell )

Immunohistochemistry (IHC)

(Anti-CACYBP antibody, MBS175645,IHC(P)IHC(P): Rat Brain Tissue )

Immunohistochemistry (IHC)

(Anti-CACYBP antibody, MBS175645,IHC(P)IHC(P): Human Liver Cancer Tissue )

Related Product Information for anti-CACYBP antibody

Description: Rabbit IgG polyclonal antibody for Calcyclin-binding protein(CACYBP) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: CACYBP(Calcyclin-binding protein), also called SIP, is a protein that in humans is encoded by the CACYBP gene. The full-length SIP cDNA encodes a predicted 228-amino acid protein. Sequence analysis of the shortest cDNA derived by 2-hybrid screening revealed an 8-amino acid difference in the deduced open reading frame followed by a stop codon, resulting in a predicted 80-amino acid protein, SIP-short(SIPS). The CACYBP gene is mapped on 1q25.1. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. The C-terminal region of SIP that is homologous to SGT1 was able to complement defects in yeast strains containing SGT1 mutant alleles, demonstrating conservation of SGT1 and SIP protein function.

NCBI and Uniprot Product Information

• NCBI GI #: 46576651
• NCBI GeneID: 27101
• UniProt Accession #: Q9HB71; O60666; Q5R370; Q5R371; B2ZWH2; B3KSF1
• Molecular Weight: 21,228 Da
• NCBI Official Full Name: Calcyclin-binding protein
• NCBI Official Synonym Full Names: calcyclin binding protein
• NCBI Official Symbol: CACYBP
• NCBI Official Synonym Symbols: SIP; GIG5; PNAS-107; S100A6BP
• NCBI Protein Information: calcyclin-binding protein; hCacyBP; S100A6-binding protein; Siah-interacting protein (SIP); growth-inhibiting gene 5 protein
• UniProt Protein Name: Calcyclin-binding protein
• Protein Family: Calcyclin-binding protein
• UniProt Gene Name: CACYBP
• UniProt Synonym Gene Names: S100A6BP; SIP; CacyBP; hCacyBP
• UniProt Entry Name: CYBP_HUMAN

NCBI Description

The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CACYBP: a nuclear/cytoplasmic protein involved in calcium-dependent ubiquitination and proteosomal degradation of target proteins. A possible molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). Interacts with proteins of the S100 family at physiological calcium concentrations. Component of some large E3 complexes and interacts directly with SIAH1, SIAH2 and SKP1A. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated. Two alternatively spliced isoforms have been described.

Protein type: Adaptor/scaffold; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q24-q25

Cellular Component: nucleoplasm; neuron projection; nuclear envelope lumen; cytoplasm; beta-catenin destruction complex

Molecular Function: protein binding; protein homodimerization activity

Biological Process: positive regulation of DNA replication; cardiac muscle cell differentiation; aging