Rabbit SHMT1 Polyclonal Antibody | anti-SHMT1 antibody

SHMT1 Rabbit Polyclonal

Cat. Num. AAA7604083

• Gene Names: SHMT1; SHMT; CSHMT
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: SHMT1; Polyclonal Antibody; SHMT1 Rabbit Polyclonal; anti-SHMT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 345

• Applicable Applications for anti-SHMT1 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Immunogen: Serine hydroxymethyltransferase 1 (soluble)
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using MBS7604083 (SHMT1 Antibody) at dilution of 1:100)

Western Blot (WB)

(HeLa cells were subjected to SDS PAGE followed by western blot with MBS7604083 (SHMT1 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 528881075
• NCBI GeneID: 6470
• NCBI Accession #: NP_001268715.1
• NCBI GenBank Nucleotide #: NM_001281786.1
• Molecular Weight: 37,672 Da
• NCBI Official Full Name: serine hydroxymethyltransferase, cytosolic isoform 3
• NCBI Official Synonym Full Names: serine hydroxymethyltransferase 1
• NCBI Official Symbol: SHMT1
• NCBI Official Synonym Symbols: SHMT; CSHMT
• NCBI Protein Information: serine hydroxymethyltransferase, cytosolic
• UniProt Protein Name: Serine hydroxymethyltransferase, cytosolic
• Protein Family: Serine hydroxymethyltransferase
• UniProt Gene Name: SHMT1
• UniProt Synonym Gene Names: SHMT
• UniProt Entry Name: GLYC_HUMAN

NCBI Description

This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

SHMT1: Interconversion of serine and glycine. Belongs to the SHMT family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; Lyase; EC 2.1.2.1; Mitochondrial; Energy Metabolism - methane; Amino Acid Metabolism - glycine, serine and threonine; Other Amino Acids Metabolism - cyanoamino acid; Methyltransferase

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: cytoplasm; cytosol; mitochondrion; nucleus

Molecular Function: glycine hydroxymethyltransferase activity; L-allo-threonine aldolase activity; protein binding; protein homodimerization activity; pyridoxal phosphate binding

Biological Process: carnitine biosynthetic process; dTMP biosynthetic process; folic acid metabolic process; glycine biosynthetic process from serine; glycine metabolic process; L-serine catabolic process; L-serine metabolic process; protein homotetramerization; protein tetramerization; purine base biosynthetic process; tetrahydrofolate metabolic process