Rabbit anti-Human SHMT1 Polyclonal Antibody | anti-SHMT1 antibody

SHMT1, NT (SHMT1, Serine hydroxymethyltransferase, cytosolic, Glycine hydroxymethyltransferase, Serine methylase) (Biotin)

Cat. Num. AAA6347452

• Gene Names: SHMT1; SHMT; CSHMT
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SHMT1; Polyclonal Antibody; NT (SHMT1; Serine hydroxymethyltransferase; cytosolic; Glycine hydroxymethyltransferase; Serine methylase) (Biotin); Serine methylase; anti-SHMT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SHMT1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SHMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-47 amino acids from the N-terminal region of human SHMT1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SHMT1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SHMT1 antibody detected the SHMT1 protein (arrow).)

Immunohistochemistry (IHC)

(SHMT1 Antibody (N-term) (AP14423a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SHMT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SHMT1 antibody

This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.

Product Categories/Family for anti-SHMT1 antibody

Antibodies; Amino Acids

References

Porter, K.E., et al. Environ. Res. 110(6):580-587(2010). Summers, C.M., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(8):679-688(2010). Vijayakrishnan, J., et al. Haematologica 95(8):1405-1414(2010). Levine, A.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1812-1821(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 22547186
• NCBI GeneID: 6470
• NCBI Accession #: NP_004160.3; NP_683718.1
• NCBI GenBank Nucleotide #: NM_004169.4
• UniProt Accession #: P34896; Q96HY0; Q9UMD1; Q9UMD2; B4DPM9; D3DXD0
• Molecular Weight: 37,672 Da
• NCBI Official Full Name: serine hydroxymethyltransferase, cytosolic isoform 1
• NCBI Official Synonym Full Names: serine hydroxymethyltransferase 1 (soluble)
• NCBI Official Symbol: SHMT1
• NCBI Official Synonym Symbols: SHMT; CSHMT
• NCBI Protein Information: serine hydroxymethyltransferase, cytosolic; serine methylase; glycine hydroxymethyltransferase; cytoplasmic serine hydroxymethyltransferase
• UniProt Protein Name: Serine hydroxymethyltransferase, cytosolic
• Protein Family: Serine hydroxymethyltransferase
• UniProt Gene Name: SHMT1
• UniProt Synonym Gene Names: SHMT
• UniProt Entry Name: GLYC_HUMAN

NCBI Description

This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

SHMT1: Interconversion of serine and glycine. Belongs to the SHMT family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 2.1.2.1; Amino Acid Metabolism - glycine, serine and threonine; Energy Metabolism - methane; Other Amino Acids Metabolism - cyanoamino acid; Lyase; Methyltransferase; Mitochondrial

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: L-allo-threonine aldolase activity; amino acid binding; protein homodimerization activity; glycine hydroxymethyltransferase activity; pyridoxal phosphate binding

Biological Process: purine base biosynthetic process; vitamin metabolic process; folic acid metabolic process; L-serine catabolic process; glycine biosynthetic process from serine; water-soluble vitamin metabolic process; carnitine biosynthetic process; protein tetramerization; protein homotetramerization