Western Blot (WB)
(Western Blot analysis of HepG2 cells using Phospho-Shc (Tyr427) Polyclonal Antibody at dilution of 1:500.)
Rabbit Shc Polyclonal Antibody | anti-Shc antibody
Phospho-Shc (Y427) Polyclonal Antibody
Western Blot (WB)
(Western Blot analysis of HepG2 cells using Phospho-Shc (Tyr427) Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of HepG2 cells using Phospho-Shc (Tyr427) Polyclonal Antibody at dilution of 1:500.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
Uniprot Description
SHC1: an adaptor protein containing a SH2 domain and a PID domain within a PH domain-like fold. Couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Six human isoforms are produced by alternative promoter usage and alternative splicing. Isoforms p66, p52 and p46 (P29353-1, -2, and -3), produced by alternative initiation, variously regulate growth factor signaling, oncogenesis, intracellular oxidant levels, and apoptosis. Isoforms p46 and p52, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex, thus initiating the cytoplasmic proliferative Ras signaling cascade in various non-neuronal systems. Isoform p66 does not mediate Ras activation, but associates with mitochondria where it controls intracellular redox status, mitochondrial permeability, life span, and stress-induced apoptosis. p66 acts as a downstream target of the tumor suppressor p53 and is required for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. P66 deletion in mice decreases the incidence of aging-associated diseases, such as atherosclerosis, and significantly prolongs life span. Participates in signaling downstream of TIE2, the tyrosine kinase receptor for angiopoietin, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. Interacts with tyrosine-phosphorylated CD3T, DDR2, LRP1, IRS4, SHP, FLT4, PDGFRB, TIE2, TrkA, -B and -C. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR in vitro via the PID domain. p66Shc is known to be activated by the mutant SOD1 associated with familial forms of amyotrophic lateral sclerosis (ALS), causing a decrease in the activity of Rac1 through a redox-sensitive regulation. In case of oxidative conditions, phosphorylation at S36 of isoform p66Shc, leads to mitochondrial accumulation p66 plays a role in mediating mitophagy and determining neuronal cell fate following acute oxygen glucose deprivation. Isoform p46 is localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.
Protein type: Motility/polarity/chemotaxis; Mitochondrial; Apoptosis; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: cytosol; endosome membrane; mitochondrial matrix; nucleus; plasma membrane
Molecular Function: ephrin receptor binding; epidermal growth factor receptor binding; insulin receptor binding; insulin-like growth factor receptor binding; neurotrophin TRKA receptor binding; phospholipid binding; phosphotyrosine binding; protein binding; protein phosphatase 2A binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase adaptor protein activity
Biological Process: actin cytoskeleton reorganization; activation of MAPK activity; activation of MAPKK activity; aging; angiogenesis; axon guidance; blood coagulation; cell-cell adhesion; cellular protein metabolic process; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; heart development; innate immune response; insulin receptor signaling pathway; leukocyte migration; MAPKKK cascade; nerve growth factor receptor signaling pathway; organ regeneration; peptidyl-tyrosine phosphorylation; platelet activation; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of smooth muscle cell proliferation; positive regulation of vasoconstriction; Ras protein signal transduction; regulation of epidermal growth factor receptor activity; regulation of growth; response to glucocorticoid stimulus; response to hydrogen peroxide; response to hypoxia; response to nicotine; response to toxin; small GTPase mediated signal transduction; unfolded protein response; vascular endothelial growth factor receptor signaling pathway; viral reproduction
Research Articles on Shc
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Product Notes
The Shc shc1 (Catalog #AAA2537219) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Shc (Y427) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Shc can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000, IHC: 1:50-1:200, ELISA: 1:5000-1:20000. Researchers should empirically determine the suitability of the Shc shc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Shc, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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