Rabbit anti-Human, Mouse Shb Polyclonal Antibody | anti-Shb antibody

Shb (Phospho-Y246) Polyclonal Antibody

Cat. Num. AAA3005359

• Gene Names: SHB; bA3J10.2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Shb; Polyclonal Antibody; Shb (Phospho-Y246) Polyclonal Antibody; SH2 domain-containing adapter protein B; SHB; anti-Shb antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-Shb (Y246) polyclonal antibody detects endogenous levels of Shb protein only when phosphorylated at Tyr246.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 509

• Applicable Applications for anti-Shb antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human Shb around the phosphorylation site of Tyrosine 246.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Related Product Information for anti-Shb antibody

The SH2 (Src Homology 2) domain is a structurally conserved motif that contains two alpha helices and seven beta strands and is found in a variety of proteins that are involved in signal transduction throughout the cell. Specifically, the SH2 domain targets SH2 domain-containing proteins to tyrosinephosphorylated sites, an event that can trigger a protein-protein interaction cascade which may ultimately effect gene expression and cellular function. Shb (SH2 domain-containing adapter protein b), Shd (SH2 domain-containing adapter protein d), She (SH2 domain-containing adapter protein e) and Shf(SH2 domain-containing adapter protein f) are SH2 domain-containing proteins that play various roles throughout the cell.

NCBI and Uniprot Product Information

• NCBI GI #: 106879210
• NCBI GeneID: 6461
• NCBI Accession #: NP_003019.2
• NCBI GenBank Nucleotide #: NP_003019.2
• UniProt Accession #: Q15464
• Molecular Weight: ~55kDa
• NCBI Official Full Name: SH2 domain-containing adapter protein B
• NCBI Official Synonym Full Names: SH2 domain containing adaptor protein B
• NCBI Official Symbol: SHB
• NCBI Official Synonym Symbols: bA3J10.2
• NCBI Protein Information: SH2 domain-containing adapter protein B
• UniProt Protein Name: SH2 domain-containing adapter protein B
• Protein Family: SH2 domain-containing adapter protein
• UniProt Gene Name: SHB
• UniProt Entry Name: SHB_HUMAN

Uniprot Description

SHB: Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic- FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9p13.2

Cellular Component: plasma membrane; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity

Biological Process: positive regulation of signal transduction; apoptosis; angiogenesis; signal transduction; cell differentiation; vascular endothelial growth factor receptor signaling pathway