Rabbit anti-Mouse SHANK2 Polyclonal Antibody | anti-SHANK2 antibody

SHANK2, ID (SHANK2, CORTBP1, KIAA1022, PROSAP1, SH3 and multiple ankyrin repeat domains protein 2, Cortactin-binding protein 1, Proline-rich synapse-associated protein 1) (FITC)

Cat. Num. AAA6347310

• Gene Names: SHANK2; SHANK; AUTS17; CORTBP1; CTTNBP1; ProSAP1; SPANK-3
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: SHANK2; Polyclonal Antibody; ID (SHANK2; CORTBP1; KIAA1022; PROSAP1; SH3 and multiple ankyrin repeat domains protein 2; Cortactin-binding protein 1; Proline-rich synapse-associated protein 1) (FITC); Proline-rich synapse-associated protein 1; anti-SHANK2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse SHANK2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SHANK2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 886-916 of human SHANK2. UniProt: SwissPort: Q9UPX8.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SHANK2 Antibody (Center) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the SHANK2 antibody detected the SHANK2 protein (arrow).)

Product Categories/Family for anti-SHANK2 antibody

Antibodies; Binding Proteins

References

1. Pinto, D., et al. Nature 466(7304):368-372(2010). 2. Berkel, S., et al. Nat. Genet. 42(6):489-491(2010). 3. Lee, J.S., et al. J. Biol. Chem. 285(11):8104-8113(2010). 4. Wu, C., et al. Proteomics 7(11):1775-1785(2007). 5. Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 226817313
• NCBI GeneID: 22941
• NCBI Accession #: NP_036441.2
• NCBI GenBank Nucleotide #: NM_012309.3
• UniProt Accession #: Q9UPX8; Q3Y8G9; Q52LK2; Q9UKP1; C0SPG8; C0SPG9
• Molecular Weight: 158,822 Da
• NCBI Official Full Name: SH3 and multiple ankyrin repeat domains protein 2 isoform 1
• NCBI Official Synonym Full Names: SH3 and multiple ankyrin repeat domains 2
• NCBI Official Symbol: SHANK2
• NCBI Official Synonym Symbols: SHANK; AUTS17; CORTBP1; CTTNBP1; ProSAP1; SPANK-3
• NCBI Protein Information: SH3 and multiple ankyrin repeat domains protein 2; cortactin-binding protein 1; GKAP/SAPAP interacting protein; cortactin SH3 domain-binding protein; proline-rich synapse associated protein 1; proline-rich synapse-associated protein 1
• UniProt Protein Name: SH3 and multiple ankyrin repeat domains protein 2
• Protein Family: SH3 and multiple ankyrin repeat domains protein
• UniProt Gene Name: SHANK2
• UniProt Synonym Gene Names: CORTBP1; KIAA1022; PROSAP1; Shank2; CortBP1
• UniProt Entry Name: SHAN2_HUMAN

NCBI Description

This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density (PSD). Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, an SH3 domain, a PSD-95/Dlg/ZO-1 domain, a sterile alpha motif domain, and a proline-rich region. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain. Two alternative splice variants, encoding distinct isoforms, are reported. Additional splice variants exist but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

SHANK2 iso4: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. Defects in SHANK2 are a cause of susceptibility to autism type 17 (AUTS17). Autism is a complex multifactorial, pervasive developmental disorder characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Most individuals with autism also manifest moderate mental retardation. Belongs to the SHANK family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: postsynaptic membrane; neuron projection; photoreceptor inner segment; growth cone; photoreceptor outer segment; cell soma; brush border membrane; apical plasma membrane; postsynaptic density; plasma membrane; neurofilament; dendritic spine; ionotropic glutamate receptor complex; cell junction

Molecular Function: ionotropic glutamate receptor binding; protein binding; GKAP/Homer scaffold activity; SH3 domain binding

Biological Process: synaptogenesis; adult behavior; social behavior; learning; memory

Disease: Autism, Susceptibility To, 17