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Flow Cytometry (FC/FACS) (Flow Cytometric analysis of Hela cells using VWA5A antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Mouse VWA5A Monoclonal Antibody | anti-VWA5A antibody

VWA5A antibody

Gene Names
VWA5A; BCSC1; BCSC-1; LOH11CR2A
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
VWA5A antibody was purified by affinity chromatography.
Synonyms
VWA5A; Monoclonal Antibody; VWA5A antibody; Monoclonal VWA5A; Anti-VWA5A; von Willebrand factor A domain containing 5A; BCSC1; LOH11CR2A; Breast cancer suppressor candidate 1; Ortholog of mouse AW551984; VWAA 5; VWAA-5; anti-VWA5A antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3D6
Purity/Purification
VWA5A antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
786
Applicable Applications for anti-VWA5A antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
IHC: 1:150
WB: 1:2000
Biological Significance
VWA5A may play a role in tumorigenesis as a tumor suppressor. Altered expression of this protein and disruption of the molecular pathway it is involved in, may contribute directly to or modify tumorigenesis.
Cross-Reactivity
Human
Immunogen
VWA5A antibody was raised in mouse using a full length recombinant protein of human VWA5A (NP_055437) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells using VWA5A antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Flow Cytometry (FC/FACS) (Flow Cytometric analysis of Hela cells using VWA5A antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant VWA5A protein (Right) or empty vector (Left) detected with VWA5A antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant VWA5A protein (Right) or empty vector (Left) detected with VWA5A antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of VWA5A protein in paraffin embedded Carcinoma of Human lung tissue using VWA5A antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of VWA5A protein in paraffin embedded Carcinoma of Human lung tissue using VWA5A antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant VWA5A protein using VWA5A antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant VWA5A protein using VWA5A antibody)
Related Product Information for anti-VWA5A antibody
Mouse monoclonal VWA5A antibody
Product Categories/Family for anti-VWA5A antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,235 Da
NCBI Official Full Name
von Willebrand factor A domain-containing protein 5A isoform 1
NCBI Official Synonym Full Names
von Willebrand factor A domain containing 5A
NCBI Official Symbol
VWA5A
NCBI Official Synonym Symbols
BCSC1; BCSC-1; LOH11CR2A
NCBI Protein Information
von Willebrand factor A domain-containing protein 5A
UniProt Protein Name
von Willebrand factor A domain-containing protein 5A
UniProt Gene Name
VWA5A
UniProt Synonym Gene Names
BCSC1; LOH11CR2A; BCSC-1
UniProt Entry Name
VMA5A_HUMAN

Uniprot Description

VWA5A: May play a role in tumorigenesis as a tumor suppressor. Altered expression of this protein and disruption of the molecular pathway it is involved in, may contribute directly to or modify tumorigenesis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 11q24.1

Cellular Component: nucleoplasm; nucleus

Research Articles on VWA5A

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Product Notes

The VWA5A vwa5a (Catalog #AAA834127) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's VWA5A can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC: 1:100 IF: 1:100 IHC: 1:150 WB: 1:2000. Researchers should empirically determine the suitability of the VWA5A vwa5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VWA5A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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