Western Blot (WB)
(Western blot analysis of extracts from HuvEc cells, using SH3RF3 antibody.)
Rabbit anti-Human, Mouse SH3RF3 Polyclonal Antibody | anti-SH3RF3 antibody
SH3RF3 Antibody
Gene Names
SH3RF3; POSH2; SH3MD4
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SH3RF3; Polyclonal Antibody; SH3RF3 Antibody; Plenty of SH3s 2; POSH2; SH3 domain-containing RING finger protein 3; SH3 multiple domains protein 4; SH3MD4; SH3R3_HUMAN; anti-SH3RF3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
SH3RF3 antibody detects endogenous levels of total SH3RF3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
882
Applicable Applications for anti-SH3RF3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Bovine (83%), Horse (92%), Sheep (83%), Rabbit (92%), Dog (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HuvEc cells, using SH3RF3 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HuvEc cells, using SH3RF3 antibody.)
Immunofluorescene (IF)
(MBS9605958 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9605958 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-SH3RF3 antibody
Subunit Structure: Interacts with PAK2.
Similarity: The third SH3 domain mediates interaction with PAK2. Belongs to the SH3RF family.
Similarity: The third SH3 domain mediates interaction with PAK2. Belongs to the SH3RF family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 92 kD
Predicted: 93 kDa
Predicted: 93 kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase SH3RF3
NCBI Official Synonym Full Names
SH3 domain containing ring finger 3
NCBI Official Symbol
SH3RF3
NCBI Official Synonym Symbols
POSH2; SH3MD4
NCBI Protein Information
E3 ubiquitin-protein ligase SH3RF3; SH3 domain-containing RING finger protein 3
UniProt Protein Name
E3 ubiquitin-protein ligase SH3RF3
Protein Family
UniProt Gene Name
SH3RF3
Uniprot Description
Has E3 ubiquitin-protein ligase activity.
Research Articles on SH3RF3
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Product Notes
The SH3RF3 sh3rf3 (Catalog #AAA9605958) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SH3RF3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SH3RF3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the SH3RF3 sh3rf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SH3RF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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