Western Blot (WB)
(ARHGAP1 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ARHGAP1 antibody detected the ARHGAP1 protein (arrow).)
Rabbit anti-Human ARHGAP1 Polyclonal Antibody | anti-ARHGAP1 antibody
ARHGAP1, ID (ARHGAP1, CDC42GAP, RHOGAP1, Rho GTPase-activating protein 1, CDC42 GTPase-activating protein, GTPase-activating protein rhoOGAP, Rho-related small GTPase protein activator, Rho-type GTPase-activating protein 1, p50-RhoGAP) (Biotin)
Gene Names
ARHGAP1; RHOGAP; RHOGAP1; CDC42GAP; p50rhoGAP
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ARHGAP1; Polyclonal Antibody; ID (ARHGAP1; CDC42GAP; RHOGAP1; Rho GTPase-activating protein 1; CDC42 GTPase-activating protein; GTPase-activating protein rhoOGAP; Rho-related small GTPase protein activator; Rho-type GTPase-activating protein 1; p50-RhoGAP) (Biotin); p50-RhoGAP; anti-ARHGAP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-ARHGAP1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
ARHGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 125-154 amino acids selected from the Central region of human ARHGAP1.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(ARHGAP1 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ARHGAP1 antibody detected the ARHGAP1 protein (arrow).)
Western Blot (WB)
(ARHGAP1 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ARHGAP1 antibody detected the ARHGAP1 protein (arrow).)
Related Product Information for anti-ARHGAP1 antibody
GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.
Product Categories/Family for anti-ARHGAP1 antibody
References
Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010). Rivadeneira, F., et al. Nat. Genet. 41(11):1199-1206(2009). Mori, K., et al. J. Biol. Chem. 284(8):5067-5076(2009). Scholz, R.P., et al. J. Cell. Sci. 122 (PT 1), 92-102 (2009): Budanova, E.N., et al. Genetika 44(2):170-176(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,436 Da
NCBI Official Full Name
rho GTPase-activating protein 1
NCBI Official Synonym Full Names
Rho GTPase activating protein 1
NCBI Official Symbol
ARHGAP1
NCBI Official Synonym Symbols
RHOGAP; RHOGAP1; CDC42GAP; p50rhoGAP
NCBI Protein Information
rho GTPase-activating protein 1; CDC42 GTPase-activating protein; GTPase-activating protein rhoGAP; GTPase-activating protein rhoOGAP; GTPase-activating protein, Rho, 1; rho-type GTPase-activating protein 1; rho-related small GTPase protein activator
UniProt Protein Name
Rho GTPase-activating protein 1
Protein Family
UniProt Gene Name
ARHGAP1
UniProt Synonym Gene Names
CDC42GAP; RHOGAP1
UniProt Entry Name
RHG01_HUMAN
NCBI Description
This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members. [provided by RefSeq, Aug 2012]
Uniprot Description
ARHGAP1: GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.
Protein type: GAPs; Motility/polarity/chemotaxis; GAPs, Rac/Rho
Chromosomal Location of Human Ortholog: 11p11.2
Cellular Component: ruffle; plasma membrane; cytosol
Molecular Function: protein binding; SH3/SH2 adaptor activity; SH3 domain binding
Biological Process: regulation of small GTPase mediated signal transduction; positive regulation of signal transduction; small GTPase mediated signal transduction; Rho protein signal transduction
Research Articles on ARHGAP1
Similar Products
Product Notes
The ARHGAP1 arhgap1 (Catalog #AAA6274832) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARHGAP1, ID (ARHGAP1, CDC42GAP, RHOGAP1, Rho GTPase-activating protein 1, CDC42 GTPase-activating protein, GTPase-activating protein rhoOGAP, Rho-related small GTPase protein activator, Rho-type GTPase-activating protein 1, p50-RhoGAP) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ARHGAP1 arhgap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
