Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Rabbit SH3BP5 Polyclonal Antibody | anti-SH3BP5 antibody
SH3BP5 Antibody
Gene Names
SH3BP5; SAB; SH3BP-5
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
SH3BP5; Polyclonal Antibody; SH3BP5 Antibody; 3BP5_HUMAN; OTTHUMP00000208289; OTTHUMP00000208291; OTTHUMP00000208294; SAB; SH3 binding protein; SH3 domain binding protein 5 (BTK associated); SH3 domain binding protein 5; SH3 domain binding protein that preferentially associates with BTK; SH3 domain-binding protein 5; SH3 domain-binding protein that preferentially associates with BTK; SH3 domainbinding protein 5; SH3BP 5; SH3BP-5; Sh3bp5; anti-SH3BP5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
SH3BP5 Antibody detects endogenous levels of SH3BP5.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
298
Applicable Applications for anti-SH3BP5 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human SH3BP5.
Tissue Specificity
Highly expressed in testis and ovaries. It is also expressed in a variety of tissues including spleen, lymph node, thymus, bone marrow, fetal liver, colon, small intestine and prostate.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Mitochondrion
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Western Blot (WB)
(Western blot analysis of extracts from Raji cells, using SH3BP5 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Raji cells, using SH3BP5 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from B16F10 cells, using SH3BP5 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from B16F10 cells, using SH3BP5 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-SH3BP5 antibody
Description: Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death.
Subunit Structure: Interacts with BTK (PubMed:9571151, PubMed:10339589). Interacts with all isoforms of MAPK8, MAPK9, MAPK10 and MAPK12 (PubMed:12167088). Interacts with GDP-bound and nucleotide-free forms of RAB11A (PubMed:26506309).
Similarity: Belongs to the SH3BP5 family.
Subunit Structure: Interacts with BTK (PubMed:9571151, PubMed:10339589). Interacts with all isoforms of MAPK8, MAPK9, MAPK10 and MAPK12 (PubMed:12167088). Interacts with GDP-bound and nucleotide-free forms of RAB11A (PubMed:26506309).
Similarity: Belongs to the SH3BP5 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 47 kDa
Predicted: 51 kDa
Predicted: 51 kDa
NCBI Official Full Name
SH3 domain-binding protein 5 isoform b
NCBI Official Synonym Full Names
SH3 domain binding protein 5
NCBI Official Symbol
SH3BP5
NCBI Official Synonym Symbols
SAB; SH3BP-5
NCBI Protein Information
SH3 domain-binding protein 5
UniProt Protein Name
SH3 domain-binding protein 5
Protein Family
UniProt Gene Name
SH3BP5
UniProt Synonym Gene Names
SAB; SH3BP-5
UniProt Entry Name
3BP5_HUMAN
Research Articles on SH3BP5
Similar Products
Product Notes
The SH3BP5 sh3bp5 (Catalog #AAA9611489) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SH3BP5 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's SH3BP5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the SH3BP5 sh3bp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SH3BP5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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