Testing Data
(3T3-Src (Y527F) transformed cells (A) and 3T3 cells (B) were plated and fixed then stained with a 1:3000 dilution of MBS619978. Proteins were visualized using an anti-rabbit secondary conjugated to FITC (1).)
Rabbit anti-Human, Mouse SH3 and PX domain-containing protein 2B Polyclonal Antibody | anti-SH3PXD2B antibody
SH3 and PX domain-containing protein 2B (SH3PXD2B, Factor for adipocyte differentiation 49, SH3 and PX domains 2B, Factor for adipocyte differentiation 49, fad49, Fad49, G431001E03Rik)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Western Blot: 3ug/ml. 50ug of NIH3T3 IP cell lysate was resolved via SDS-PAGE, transferred to PVDF and probed with 3ug/ml S1013-33A. Proteins were visualized using an IgG (HRP) anti-rabbit that does not recognize denatured IgG. Detection using a chemiluminescence system.
Immunoprecipitation: 10ug immunoprecipitates 500ug of NIH3T3 whole cell lysate
Immunocytochemistry: 1:3000
Testing Data
(3T3-Src (Y527F) transformed cells (A) and 3T3 cells (B) were plated and fixed then stained with a 1:3000 dilution of MBS619978. Proteins were visualized using an anti-rabbit secondary conjugated to FITC (1).)
Testing Data
(3T3-Src (Y527F) transformed cells (A) and 3T3 cells (B) were plated and fixed then stained with a 1:3000 dilution of MBS619978. Proteins were visualized using an anti-rabbit secondary conjugated to FITC (1).)
Immunoprecipitation (IP)
(10ug of MBS619978 was used to immunoprecipitate from 500ug of NIH3T3 whole cell lysate. The ab was collected with Protein A beads and eluted with sample buffer. 50ug of NIH3T3 IP cell lysate was then resolved via SDS-PAGE, transferred to PVDF and probed with 3ug/ml MBS619978. Proteins were visualized using an anti-rabbit (HRP) secondary antibody that does not recognize denatured IgG. ECL detection)
Immunoprecipitation (IP)
(10ug of MBS619978 was used to immunoprecipitate from 500ug of NIH3T3 whole cell lysate. The ab was collected with Protein A beads and eluted with sample buffer. 50ug of NIH3T3 IP cell lysate was then resolved via SDS-PAGE, transferred to PVDF and probed with 3ug/ml MBS619978. Proteins were visualized using an anti-rabbit (HRP) secondary antibody that does not recognize denatured IgG. ECL detection)
SH3PXD2B is broadly expressed and localizes in the cytoplasm of the podosome.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an adapter protein that is characterized by a PX domain and four Src homology 3 domains. The encoded protein is required for podosome formation and is involved in cell adhesion and migration of numerous cell types. Mutations in this gene are the cause of Frank-ter Haar syndrome. [provided by RefSeq]
Uniprot Description
SH3PXD2B: Adapter protein involved in invadopodia and podosome formation and extracellular matrix degradation. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. Plays a role in mitotic clonal expansion during the immediate early stage of adipocyte differentiation. Defects in SH3PXD2B are the cause of Frank-Ter Haar syndrome (FTHS). It is a syndrome characterized by brachycephaly, wide fontanels, prominent forehead, hypertelorism, prominent eyes, macrocornea with or without glaucoma, full cheeks, small chin, bowing of the long bones and flexion deformity of the fingers. Belongs to the SH3PXD2 family.
Protein type: Lipid-binding
Chromosomal Location of Human Ortholog: 5q35.1
Cellular Component: cell projection; cytoplasm; podosome; cell junction
Molecular Function: protein binding; phosphatidylinositol-5-phosphate binding; SH2 domain binding; phosphatidylinositol 3-phosphate binding
Biological Process: extracellular matrix disassembly; eye development; heart development; superoxide metabolic process; positive regulation of fat cell differentiation; cell differentiation; skeletal development
Disease: Frank-ter Haar Syndrome
Research Articles on SH3PXD2B
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Product Notes
The SH3PXD2B sh3pxd2b (Catalog #AAA619978) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SH3 and PX domain-containing protein 2B (SH3PXD2B, Factor for adipocyte differentiation 49, SH3 and PX domains 2B, Factor for adipocyte differentiation 49, fad49, Fad49, G431001E03Rik) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SH3 and PX domain-containing protein 2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC). Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Dilution: Western Blot: 3ug/ml. 50ug of NIH3T3 IP cell lysate was resolved via SDS-PAGE, transferred to PVDF and probed with 3ug/ml S1013-33A. Proteins were visualized using an IgG (HRP) anti-rabbit that does not recognize denatured IgG. Detection using a chemiluminescence system. Immunoprecipitation: 10ug immunoprecipitates 500ug of NIH3T3 whole cell lysate Immunocytochemistry: 1:3000. Researchers should empirically determine the suitability of the SH3PXD2B sh3pxd2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SH3 and PX domain-containing protein 2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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