Testing Data
(3T3-Src (Y527F) transformed cells (A) and 3T3 cells (B) were plated and fixed then stained with a 1:3000 dilution ofMBS619978. Proteins were visualized using an anti-rabbit secondary conjugated to FITC (1).)
Rabbit anti-Mouse, Human SH3 and PX domain-containing protein 2B Polyclonal Antibody | anti-SH3PXD2B antibody
SH3 and PX domain-containing protein 2B (SH3PXD2B, Factor for adipocyte differentiation 49, SH3 and PX domains 2B, Factor for adipocyte differentiation 49, fad49, Fad49, G431001E03Rik) (APC)
Gene Names
Sh3pxd2b; TSK4; fad49; G431001E03Rik
Reactivity
Mouse, Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SH3 and PX domain-containing protein 2B; Polyclonal Antibody; SH3 and PX domain-containing protein 2B (SH3PXD2B; Factor for adipocyte differentiation 49; SH3 and PX domains 2B; fad49; Fad49; G431001E03Rik) (APC); anti-SH3PXD2B antibody
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse SH3PXD2B at ~130kD. Species Crossreactivity: human. Species Sequence Homology: rat (96%), equine (88%), canine (87%) and bovine (87%).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-SH3PXD2B antibody
Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
ICC: 1:3000
IP: 10ug immunoprecipitates 500ug of NIH3T3 whole cell lysate
Applications are based on unconjugated antibody.
WB: 3ug/ml. 50ug of NIH3T3 IP cell lysate was resolved via SDS-PAGE, transferred to PVDF and probed with. Detection using a chemiluminescence system.
IP: 10ug immunoprecipitates 500ug of NIH3T3 whole cell lysate
Applications are based on unconjugated antibody.
WB: 3ug/ml. 50ug of NIH3T3 IP cell lysate was resolved via SDS-PAGE, transferred to PVDF and probed with. Detection using a chemiluminescence system.
Immunogen
Recombinant protein containing aa257-477 of mouse SH3PXD2B. Cellular Localization: Cytoplasm.
Conjugate
APC
Positive Control
NIH3T3 cell lysate
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(3T3-Src (Y527F) transformed cells (A) and 3T3 cells (B) were plated and fixed then stained with a 1:3000 dilution ofMBS619978. Proteins were visualized using an anti-rabbit secondary conjugated to FITC (1).)
Testing Data
(3T3-Src (Y527F) transformed cells (A) and 3T3 cells (B) were plated and fixed then stained with a 1:3000 dilution ofMBS619978. Proteins were visualized using an anti-rabbit secondary conjugated to FITC (1).)
Immunoprecipitation (IP)
(10ug of MBS619978 was used to immunoprecipitate from 500ug of NIH3T3 whole cell lysate. The ab was collected with Protein A beads and eluted with sample buffer. 50ug of NIH3T3 IP cell lysate was then resolved via SDS-PAGE, transferred to PVDF and probed with 3ug/mlMBS619978. Proteins were visualized using an anti-rabbit (HRP) secondary antibody that does not recognize denatured IgG. ECL detection)
Immunoprecipitation (IP)
(10ug of MBS619978 was used to immunoprecipitate from 500ug of NIH3T3 whole cell lysate. The ab was collected with Protein A beads and eluted with sample buffer. 50ug of NIH3T3 IP cell lysate was then resolved via SDS-PAGE, transferred to PVDF and probed with 3ug/mlMBS619978. Proteins were visualized using an anti-rabbit (HRP) secondary antibody that does not recognize denatured IgG. ECL detection)
Product Categories/Family for anti-SH3PXD2B antibody
References
1. Buschman, M.D., et al., (2009). Mol. Cell Biol. 20(5):1302-1311. 2. Courtneidge, S.A., et al., (2005). Cold Spring Harb Symp Quant Biol. 70:167-171.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
101,517 Da
NCBI Official Full Name
SH3 and PX domain-containing protein 2B
NCBI Official Synonym Full Names
SH3 and PX domains 2B
NCBI Official Symbol
Sh3pxd2b
NCBI Official Synonym Symbols
TSK4; fad49; G431001E03Rik
NCBI Protein Information
SH3 and PX domain-containing protein 2B; factor for adipocyte differentiation 49; tyrosine kinase substrate with four SH3 domains
UniProt Protein Name
SH3 and PX domain-containing protein 2B
Protein Family
UniProt Gene Name
Sh3pxd2b
UniProt Synonym Gene Names
Fad49; Tks4
UniProt Entry Name
SPD2B_MOUSE
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Product Notes
The SH3PXD2B sh3pxd2b (Catalog #AAA6499354) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SH3 and PX domain-containing protein 2B (SH3PXD2B, Factor for adipocyte differentiation 49, SH3 and PX domains 2B, Factor for adipocyte differentiation 49, fad49, Fad49, G431001E03Rik) (APC) reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's SH3 and PX domain-containing protein 2B can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP). ICC: 1:3000 IP: 10ug immunoprecipitates 500ug of NIH3T3 whole cell lysate Applications are based on unconjugated antibody. WB: 3ug/ml. 50ug of NIH3T3 IP cell lysate was resolved via SDS-PAGE, transferred to PVDF and probed with. Detection using a chemiluminescence system. Researchers should empirically determine the suitability of the SH3PXD2B sh3pxd2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SH3 and PX domain-containing protein 2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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