Rabbit SFTPC Polyclonal Antibody | anti-SFTPC antibody

SFTPC, NT (SFTPC, SFTP2, Pulmonary surfactant-associated protein C, Pulmonary surfactant-associated proteolipid SPL(Val), SP5) (PE)

Cat. Num. AAA6346789

• Gene Names: SFTPC; SP5; SP-C; PSP-C; SFTP2; SMDP2; BRICD6
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SFTPC; Polyclonal Antibody; NT (SFTPC; SFTP2; Pulmonary surfactant-associated protein C; Pulmonary surfactant-associated proteolipid SPL(Val); SP5) (PE); SP5; anti-SFTPC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human, mouse and rat SFTPC.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 197

• Applicable Applications for anti-SFTPC antibody: Immunofluorescence (IF), FLISA, Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a fragment within amino acids 1-30 in the N-erminal region of human SFTPC, UniProt accession #P11686.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SFTPC Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the SFTPC antibody detected the SFTPC protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of SFTPC Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

Related Product Information for anti-SFTPC antibody

This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.

Product Categories/Family for anti-SFTPC antibody

Antibodies; Pulmonary Proteins

References

Wambach, J.A., et al. Pediatr. Res. 68(3):216-220(2010). Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010). Thouvenin, G., et al. Arch. Dis. Child. 95(6):449-454(2010). Crossno, P.F., et al. Chest 137(4):969-973(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 288915521
• NCBI GeneID: 6440
• NCBI Accession #: NP_001165881.1; NP_003009.2
• NCBI GenBank Nucleotide #: NP_001165881.1
• UniProt Accession #: P11686
• NCBI Official Full Name: pulmonary surfactant-associated protein C isoform 1
• NCBI Official Synonym Full Names: surfactant protein C
• NCBI Official Symbol: SFTPC
• NCBI Official Synonym Symbols: SP5; SP-C; PSP-C; SFTP2; SMDP2; BRICD6
• NCBI Protein Information: pulmonary surfactant-associated protein C
• UniProt Protein Name: Pulmonary surfactant-associated protein C
• Protein Family: Pulmonary surfactant-associated protein
• UniProt Gene Name: SFTPC
• UniProt Synonym Gene Names: SFTP2; SP-C
• UniProt Entry Name: PSPC_HUMAN

NCBI Description

This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.[provided by RefSeq, Feb 2010]

Uniprot Description

SFTPC: Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2); also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS); also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: multivesicular body; extracellular space

Molecular Function: protein binding; protein homodimerization activity

Biological Process: circadian rhythm; response to cAMP; response to hyperoxia; response to retinoic acid; response to glucocorticoid stimulus; response to glucose stimulus; response to lipopolysaccharide; respiratory gaseous exchange; protein homooligomerization; response to vitamin A

Disease: Pulmonary Fibrosis, Idiopathic; Surfactant Metabolism Dysfunction, Pulmonary, 2