Rabbit KVbeta.3 Polyclonal Antibody | anti-KVbeta.3 antibody

KVbeta.3 Polyclonal Antibody

Cat. Num. AAA3005954

• Gene Names: KCNAB3; AKR6A9; KCNA3B; KCNA3.1B; KV-BETA-3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: KVbeta.3; Polyclonal Antibody; KVbeta.3 Polyclonal Antibody; Voltage-gated potassium channel subunit beta-3; K(+) channel subunit beta-3; Kv-beta-3; KCNAB3; KCNA3B; KV-beta.3; anti-KVbeta.3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KVbeta.3 polyclonal antibody detects endogenous levels of KVbeta.3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 404

• Applicable Applications for anti-KVbeta.3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 337-382 of Human KVbeta.3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of KVbeta. 3 polyclonal antibody at 1:500 dilutionLane1:THP-1 cell lysateLane2:Rat brain tissue lysateLane3:Rat kidney tissue lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of KVbeta. 3 pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cell membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-KVbeta.3 antibody

Voltage-gated K+ channels in the plasma membrane control the repolarization and the frequency of action potentials in neurons, muscles and other excitable cells. The KV gene family encodes more than 30 proteins that comprise the subunits of the K+ channels, and they vary in their gating and permeation properties, subcellular distribution and expression patterns. Functional KV channels assemble as tetramers consisting of pore-forming alpha subunits (KV), which include the KV1, KV2, KV3 and KV4 proteins, and accessory or KV-subunits that modify the gating properties of the coexpressed KV subunits. KVbeta.3 is an accessory K+ channel protein which regulates the activity of the pore-forming alpha subunit and alters the functional properties of Kv1.5. KVbeta.3 localizes to the cytoplasm and is expressed in the brain, with highest expression detected in the cerebellum, and weakest expression seen in the frontal and temporal lobes. No KVbeta.3 expression is detected in the heart, spinal cord, lung, liver, kidney, pancreas, placenta or skeletal muscle.

NCBI and Uniprot Product Information

• NCBI GI #: 27436971
• NCBI GeneID: 9196
• NCBI Accession #: NP_004723.2
• NCBI GenBank Nucleotide #: NP_004723.2
• UniProt Accession #: O43448
• Molecular Weight: ~ 44kDa
• NCBI Official Full Name: voltage-gated potassium channel subunit beta-3
• NCBI Official Synonym Full Names: potassium voltage-gated channel subfamily A regulatory beta subunit 3
• NCBI Official Symbol: KCNAB3
• NCBI Official Synonym Symbols: AKR6A9; KCNA3B; KCNA3.1B; KV-BETA-3
• NCBI Protein Information: voltage-gated potassium channel subunit beta-3
• UniProt Protein Name: Voltage-gated potassium channel subunit beta-3
• UniProt Gene Name: KCNAB3
• UniProt Synonym Gene Names: KCNA3B
• UniProt Entry Name: KCAB3_HUMAN

NCBI Description

This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. The encoded protein is one of the beta subunits, which are auxiliary proteins associating with functional Kv-alpha subunits. The encoded protein forms a heterodimer with the potassium voltage-gated channel, shaker-related subfamily, member 5 gene product and regulates the activity of the alpha subunit. [provided by RefSeq, May 2012]

Uniprot Description

KCNAB3: Accessory potassium channel protein which modulates the activity of the pore-forming alpha subunit. Alters the functional properties of Kv1.5. Belongs to the shaker potassium channel beta subunit family.

Protein type: Channel, potassium

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasm; integral to membrane; plasma membrane

Molecular Function: voltage-gated potassium channel activity; potassium channel regulator activity

Biological Process: synaptic transmission; potassium ion transport