Human Folliculin (FLCN) ELISA Kit | FLCN elisa kit

Human Folliculin (FLCN) ELISA Kit

Cat. Num. AAA2022660

• Gene Names: Flcn; Bhd; FLCL; AU014660; B430214A04Rik
• Reactivity: Human
• Synonyms: Folliculin (FLCN); Human Folliculin (FLCN) ELISA Kit; BHD; FLCL; BHD skin lesion fibrofolliculoma protein; Birt-Hogg-Dube syndrome protein; FLCN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Folliculin (FLCN).; No significant cross-reactivity or interference between Folliculin (FLCN) and analogues was observed.
• Sequence Length: 579

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.112ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Folliculin (FLCN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Folliculin (FLCN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLCN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Folliculin (FLCN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Folliculin (FLCN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Folliculin (FLCN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Folliculin (FLCN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FLCN elisa kit

Endocrinology

NCBI and Uniprot Product Information

• NCBI GI #: 405778337
• NCBI GeneID: 216805
• NCBI Accession #: NP_001258286.1
• NCBI GenBank Nucleotide #: NM_001271357.1
• UniProt Accession #: Q8QZS3; Q3U4U8; Q3UFZ1; Q5SWZ2; Q5SX01; Q5SX02; Q8CAC0
• Molecular Weight: 64,327 Da
• NCBI Official Full Name: folliculin
• NCBI Official Synonym Full Names: folliculin
• NCBI Official Symbol: Flcn
• NCBI Official Synonym Symbols: Bhd; FLCL; AU014660; B430214A04Rik
• NCBI Protein Information: folliculin
• UniProt Protein Name: Folliculin
• Protein Family: Folliculin
• UniProt Gene Name: Flcn
• UniProt Entry Name: FLCN_MOUSE

Uniprot Description

FLCN: a protein that is expressed in most tissues and that has no significant homology to other proteins. Involved in nutrient sensing through the AMPK and mTOR signaling pathways. Regulates lysosome function by promoting the mTORC1-dependent sequestration of transcription factor EB (TFEB). Required for the recruitment of mTORC1 to lysosomes by Rag GTPases. Amino acid depletion leads to its recruitment to the surface of lysosomes where it directly binds RagA, an interaction that is promoted by FNIP1. May be a tumor suppressor. Loss of function mutations cause Birt-Hogg-Dube syndrome (BHD), a disease characterized by fibrofolliculomas (hair follicle tumors), and predispositions for renal carcinoma, pneumothorax, and pulmonary cysts. These symptoms are similar to those that occur in TSC or tuberous sclerosis complex, suggesting the involvement in the same pathways as TSC1. Belongs to the folliculin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor

Cellular Component: cytoplasm; plasma membrane; midbody; nucleus

Molecular Function: protein binding; protein complex binding

Biological Process: intercellular junction assembly; negative regulation of TOR signaling pathway; regulation of protein amino acid phosphorylation; TOR signaling pathway; negative regulation of protein kinase B signaling cascade; positive regulation of cell adhesion; in utero embryonic development; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of histone acetylation; negative regulation of transcription from RNA polymerase II promoter; regulation of cytokinesis; regulation of TOR signaling pathway; negative regulation of angiogenesis; negative regulation of Rho protein signal transduction; positive regulation of transcription from RNA polymerase II promoter; hemopoiesis; negative regulation of cell growth; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; negative regulation of cell migration; positive regulation of TOR signaling pathway