Rabbit anti-Human SF3B1 Polyclonal Antibody | anti-SF3B1 antibody

SF3B1, NT (SF3B1, SAP155, Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155kD subunit, Spliceosome-associated protein 155) (Azide free) (HRP)

Cat. Num. AAA6346530

• Gene Names: SF3B1; MDS; PRP10; Hsh155; PRPF10; SAP155; SF3b155
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SF3B1; Polyclonal Antibody; NT (SF3B1; SAP155; Splicing factor 3B subunit 1; Pre-mRNA-splicing factor SF3b 155kD subunit; Spliceosome-associated protein 155) (Azide free) (HRP); Spliceosome-associated protein 155; anti-SF3B1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SF3B1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SF3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-402 amino acids from the N-terminal region of human SF3B1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SF3B1 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SF3B1 antibody detected the SF3B1 protein (arrow).)

Immunohistochemistry (IHC)

(SF3B1 Antibody (N-term) (AP13754a)immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SF3B1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SF3B1 antibody

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

Product Categories/Family for anti-SF3B1 antibody

Antibodies; Splicing Factors

References

Corsini, L., et al. J. Biol. Chem. 284(1):630-639(2009). Tanuma, N., et al. J. Biol. Chem. 283(51):35805-35814(2008). Pessa, H.K., et al. Proc. Natl. Acad. Sci. U.S.A. 105(25):8655-8660(2008). Kuwasako, K., et al. Proteins 71(4):1617-1636(2008). Avila, M.L., et al. Biochem. Biophys. Res. Commun. 364(1):26-32(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 54112117
• NCBI GeneID: 23451
• NCBI Accession #: NP_036565.2
• NCBI GenBank Nucleotide #: NM_012433.2
• UniProt Accession #: O75533; E9PCH3
• Molecular Weight: 16,020 Da
• NCBI Official Full Name: splicing factor 3B subunit 1 isoform 1
• NCBI Official Synonym Full Names: splicing factor 3b, subunit 1, 155kDa
• NCBI Official Symbol: SF3B1
• NCBI Official Synonym Symbols: MDS; PRP10; Hsh155; PRPF10; SAP155; SF3b155
• NCBI Protein Information: splicing factor 3B subunit 1; SAP 155; pre-mRNA processing 10; spliceosome-associated protein 155; splicing factor 3b, subunit 1, 155kD; pre-mRNA splicing factor SF3b, 155 kDa subunit
• UniProt Protein Name: Splicing factor 3B subunit 1
• Protein Family: Splicing factor
• UniProt Gene Name: SF3B1
• UniProt Synonym Gene Names: SAP155; SF3b155; SAP 155
• UniProt Entry Name: SF3B1_HUMAN

NCBI Description

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

SF3B1: subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (bps) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Phosphorylated during the splicing catalytic steps.

Protein type: Spliceosome; RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: nucleoplasm; spliceosome; nuclear speck; nucleus; U12-dependent spliceosome

Molecular Function: protein binding; chromatin binding

Biological Process: anterior/posterior pattern formation; nuclear mRNA splicing, via spliceosome; RNA splicing, via transesterification reactions; RNA splicing; gene expression

Disease: Myelodysplastic Syndrome