Rabbit SF1 Polyclonal Antibody | anti-SF1 antibody

SF1 (P76) Polyclonal Antibody

Cat. Num. AAA3001359

• Gene Names: SF1; BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SF1; Polyclonal Antibody; SF1 (P76) Polyclonal Antibody; Splicing factor 1; Mammalian branch point-binding protein; BBP; mBBP; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162; ZFM1; ZNF162; anti-SF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SF1 (P76) polyclonal antibody detects endogenous levels of SF1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 673

• Applicable Applications for anti-SF1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 45-98 of Human SF1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SF1 (P76) pAb at 1:1000 dilutionLane1:AML-12 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:L02 whole cell lysate (20ug) Lane4:HEK293T whole cell lysate (20ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of SF1 (P76) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-SF1 antibody

Steroidogenic factor-1 (SF-1) regulates multiple genes involved in the adrenal and gonadal development and in the biosynthesis of a variety of hormones, including adrenal and gonadal steroids, anti-Mullerian hormone (AMH), and gonadotropins. SF-1 belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors. In the adult ovary, SF-1 localizes to theca/interstitial cells.

NCBI and Uniprot Product Information

• NCBI GI #: 295842330
• NCBI GeneID: 7536
• NCBI Accession #: NP_001171501.1
• NCBI GenBank Nucleotide #: NP_001171501.1
• UniProt Accession #: Q15637
• Molecular Weight: ~ 68kDa
• NCBI Official Full Name: splicing factor 1 isoform 6
• NCBI Official Synonym Full Names: splicing factor 1
• NCBI Official Symbol: SF1
• NCBI Official Synonym Symbols: BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25
• NCBI Protein Information: splicing factor 1
• UniProt Protein Name: Splicing factor 1
• Protein Family: Splicing factor
• UniProt Gene Name: SF1
• UniProt Synonym Gene Names: ZFM1; ZNF162; BBP; mBBP
• UniProt Entry Name: SF01_HUMAN

NCBI Description

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Uniprot Description

SF1: a splicing factor necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor. Phosphorylation of SF1 by cGMP-dependent protein kinase regulates spliceosome assembly. Binds U2AF2. Interacts with U1 snRNA. Binds EWSR1, FUS and TAF15. Six alternatively spliced isoforms have been described.

Protein type: RNA splicing; RNA-binding; Transcription, coactivator/corepressor; Spliceosome

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; spliceosome; ribosome; nucleus

Molecular Function: zinc ion binding; RNA binding; transcription corepressor activity

Biological Process: nuclear mRNA splicing, via spliceosome; nuclear mRNA 3'-splice site recognition; Leydig cell differentiation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of smooth muscle cell proliferation; male sex determination; spliceosome assembly; regulation of steroid biosynthetic process