Rabbit anti-Human, Mouse IL6ST Polyclonal Antibody | anti-IL6ST antibody

IL6ST (Phospho-S782) polyclonal antibody

Cat. Num. AAA3007866

• Gene Names: IL6ST; CD130; GP130; HIES4; CDW130; IL-6RB; sGP130
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IL6ST; Polyclonal Antibody; IL6ST (Phospho-S782) polyclonal antibody; Interleukin-6 receptor subunit beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; CDw130; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD130; anti-IL6ST antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: IL6ST (phospho-S782) polyclonal antibody detects endogenous levels of IL6ST protein only when phosphorylated at Ser782.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
• Sequence Length: 857

• Applicable Applications for anti-IL6ST antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human IL6ST around the phosphorylation site of Serine782.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of IL6ST (phospho-S782) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:L02 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))

Related Product Information for anti-IL6ST antibody

IL-6 activates intracellular signaling through binding a receptor consisting of a ligand-binding protein (IL-6R) and a second protein. IL-6 first binds to IL-6R which subsequently associates with a gp130 dimer. The active signaling complex consists of at minimum IL-6, IL-6R and a dimer of two gp130 proteins that are linked by a disulfide bond. A soluble form of IL-6R is generated by proteolytic cleavage of the membrane-bound precursor and can function as an agonistic molecule that can actively participate in cell-to-cell signaling. The second subunit of the IL-6 complex, gp130, also functions as a component of several additional receptor complexes including leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF) and IL-11. LIF binds to the LIF receptor with low affinity and to a complex of the LIF receptor and gp130 with high affinity while OSM appears to bind to gp130 with low affinity and to a complex of gp130 and the LIF receptor with high affinity.

NCBI and Uniprot Product Information

• NCBI GI #: 300244535
• NCBI GeneID: 3572
• NCBI Accession #: NP_001177910.1
• NCBI GenBank Nucleotide #: NP_001177910.1
• UniProt Accession #: P40189
• Molecular Weight: ~ 130kDa
• NCBI Official Full Name: interleukin-6 receptor subunit beta isoform 3
• NCBI Official Synonym Full Names: interleukin 6 signal transducer
• NCBI Official Symbol: IL6ST
• NCBI Official Synonym Symbols: CD130; GP130; HIES4; CDW130; IL-6RB; sGP130
• NCBI Protein Information: interleukin-6 receptor subunit beta
• UniProt Protein Name: Interleukin-6 receptor subunit beta
• Protein Family: Interleukin-6 receptor
• UniProt Gene Name: IL6ST
• UniProt Synonym Gene Names: IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; gp130
• UniProt Entry Name: IL6RB_HUMAN

NCBI Description

The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]

Uniprot Description

gp130: gp130 is a ubiquitously expressed type I cytokine family receptor. The receptor systems for IL6, LIF, OSM, CNTF, IL11 AND CT1 utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not directly bind IL6. May have a role in embryonic development. Contains 5 fibronectin type III domains and 1 immunoglobulin-like C2-type domain. Two alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: extracellular space; cell soma; membrane; oncostatin-M receptor complex; dendrite; plasma membrane; extracellular region; interleukin-6 receptor complex; external side of plasma membrane

Molecular Function: interleukin-11 binding; protein homodimerization activity; interleukin-27 receptor activity; leukemia inhibitory factor receptor activity; oncostatin-M receptor activity; interleukin-6 receptor activity; interleukin-11 receptor activity; protein binding; interleukin-6 receptor binding; interleukin-6 binding; growth factor binding; ciliary neurotrophic factor receptor activity; ciliary neurotrophic factor receptor binding

Biological Process: glycogen metabolic process; viral reproduction; cytokine and chemokine mediated signaling pathway; leukemia inhibitory factor signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of osteoblast differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; regulation of Notch signaling pathway; positive regulation of acute inflammatory response; response to cytokine stimulus; positive regulation of cell proliferation; positive regulation of T cell proliferation; positive regulation of adaptive immune response; positive regulation of astrocyte differentiation; negative regulation of apoptosis