Rabbit anti-Human SF1 Polyclonal Antibody | anti-SF1 antibody

SF1 polyclonal antibody

Cat. Num. AAA3015850

• Gene Names: NR5A1; ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXY3
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Synonyms: SF1; Polyclonal Antibody; SF1 polyclonal antibody; Steroidogenic factor 1; SF-1; STF-1; hSF-1; Adrenal 4-binding protein; Fushi tarazu factor homolog 1; Nuclear receptor subfamily 5 group A member 1; Steroid hormone receptor Ad4BP; anti-SF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: SF1 polyclonal antibody detects endogenous levels of SF1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Applicable Applications for anti-SF1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000
• Immunogen: Synthetic peptide, corresponding to Human SF1.
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of SF1 polyclonal antibody at 1:1000 dilution Lane1:Hela whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:U-87MG whole cell lysate(40ug))

Related Product Information for anti-SF1 antibody

The orphan nuclear receptor, steroidogenic factor 1 (STF-1, also called Ad4BP), is encoded by the NR5A1 gene and plays an instrumental role in directing the transcriptional control of steroidogenesis. Initially identified as a tissue-specific transcriptional regulator of cytochrome P450 steroid hydroxylases, research studies of both global and tissue-specific knockout mice have demonstrated that STF-1 is required for the development of adrenal glands, gonads, ventromedial hypothalamus, and for the proper functioning of pituitary gonadotropes. Indeed, humans with mutations that render STF-1 transcriptionally inactive can present with testicular failure, ovarian failure, and adrenal insufficiency. Furthermore, dysregulation of STF-1 has been linked to diseases such as endometriosis and adrenocortical carcinoma. Like other nuclear hormone receptors, STF-1 has a modular domain structure composed of an amino-terminal zinc finger DNA-binding domain, a ligand-binding domain, a carboxy-terminal AF-2 activation domain, and a hinge region with AF-1-like activation activity. STF-1 also contains a fushi tarazu factor 1 box, which functions as an accessory DNA binding domain. STF-1 is primarily phosphorylated at Ser203, which is thought to enhance its transcriptional activity by promoting complex formation with transcriptional cofactors. In addition to phosphorylation at Ser203, STF-1 is subject to SUMO conjugation and acetylation at epsilon-amino groups of target lysine residues. Whereas SUMOylation represses STF-1 function, acetylation enhances its transcriptional activity.

NCBI and Uniprot Product Information

• NCBI GI #: 20070193
• NCBI GeneID: 2516
• NCBI Accession #: NP_004950.2
• NCBI GenBank Nucleotide #: NM_004959.4
• UniProt Accession #: Q13285; O15196; Q5T6F5
• Molecular Weight: 51,636 Da
• NCBI Official Full Name: steroidogenic factor 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 5, group A, member 1
• NCBI Official Symbol: NR5A1
• NCBI Official Synonym Symbols: ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXY3
• NCBI Protein Information: steroidogenic factor 1; STF-1; nuclear receptor AdBP4; steroidogenic factor-1; adrenal 4 binding protein; adrenal 4-binding protein; fushi tarazu factor homolog 1; steroid hormone receptor Ad4BP; steroidogenic factor 1 nuclear receptor
• UniProt Protein Name: Steroidogenic factor 1
• Protein Family: Splicing factor
• UniProt Gene Name: NR5A1
• UniProt Synonym Gene Names: AD4BP; FTZF1; SF1; SF-1; STF-1
• UniProt Entry Name: STF1_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. [provided by RefSeq, Jul 2008]

Uniprot Description

STF-1: a nuclear hormone receptor of the NR5 subfamily. Essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P-450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the Muellerian inhibiting substance (AMH) gene as well as the AHCH and STAR genes.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; ligand-dependent nuclear receptor activity; enzyme binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; double-stranded DNA binding; steroid hormone receptor activity; phospholipid binding; chromatin binding

Biological Process: transcription initiation from RNA polymerase II promoter; tissue development; intracellular receptor-mediated signaling pathway; adrenal gland development; primary sex determination; male gonad development; multicellular organismal aging; regulation of steroid biosynthetic process; cell-cell signaling; luteinization; hormone metabolic process; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; maintenance of protein localization in nucleus; cell differentiation

Disease: Premature Ovarian Failure 7; 46,xy Sex Reversal 3; Spermatogenic Failure 8