Immunoprecipitation (IP)
(SETD7 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-SETD7 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SETD7 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size: 52 kDa)
Rabbit anti-Human SETD7 Polyclonal Antibody | anti-SETD7 antibody
Anti-SETD7 Antibody, Rabbit Polyclonal
Gene Names
SETD7; KMT7; SET7; SET9; SET7/9
Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
SETD7; Polyclonal Antibody; Anti-SETD7 Antibody; Rabbit Polyclonal; SETD7/SET7/9 Antibody; Rabbit PAb; Antigen Affinity Purified; SET domain containing (lysine methyltransferase) 7; Anti-KMT7 Antibody; Anti-SET7 Antibody; Anti-SET7/9 Antibody; Anti-SET9 Antibody; anti-SETD7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SETD7/SET7/9
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SETD7 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
Immunogen
Recombinant Human SETD7/SET7/9 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human SETD7/SET7/9 (rh SETD7/SET7/9). SETD7/SET7/9 specific IgG was purified by Human SETD7/SET7/9 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunoprecipitation (IP)
(SETD7 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-SETD7 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SETD7 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size: 52 kDa)
Immunoprecipitation (IP)
(SETD7 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-SETD7 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SETD7 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size: 52 kDa)
Western Blot (WB)
(Anti-SETD7 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDaObserved band size:53 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-SETD7 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDaObserved band size:53 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-SETD7 antibody
Histone-lysine N-methyltransferase SETD7, also known as SET domain containing (lysine methyltransferase) 7, SET7/9, Histone H3-K4 methyltransferase SETD7, H3-K4-HMTase SETD7, and SETD7, is a member of the histone-lysine methyltransferase family and SET7 subfamily. SETD7 is widely expressed and expressed in pancreatic islets. SETD7 contains three MORN repeats and one SET domain. SETD7 plays a central role in the transcriptional activation of genes such as collagenase or insulin. As a protein lysine methyltransferase (PKMT), SETD7 also has methyltransferase activity toward non-histone proteins such as p53/TP53, TAF1, and possibly TAF7 by recognizing and binding in substrate proteins. The mono-methyltransferase activity of SETD7 is achieved by disrupting the formation at near-attack conformations for the dimethylation reaction. SETD7 is also a novel coactivator of NF-kappaB and plays a role in inflammation and diabetes.
References
Wang, H. et al., 2002, Mol Cell 8 (6): 1207-17. Jacobs, SA. et al., 2002, Nat. Struct. Biol. 9 (11): 833-8. Xiao B, et al., 2003, Nature. 421 (6923): 652-6. Martens, JH. et al., 2003, Mol. Cell. Biol. 23: 1808-1816. Couture, JF. et al., 2006, Nat Struct Mol Biol 13 (2): 140-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,721 Da
NCBI Official Full Name
Homo sapiens SET domain containing (lysine methyltransferase) 7 (SETD7), mRNA
NCBI Official Synonym Full Names
SET domain containing (lysine methyltransferase) 7
NCBI Official Symbol
SETD7
NCBI Official Synonym Symbols
KMT7; SET7; SET9; SET7/9
NCBI Protein Information
histone-lysine N-methyltransferase SETD7; H3-K4-HMTase SETD7; SET domain-containing protein 7; histone H3-K4 methyltransferase SETD7; histone H3-lysine 4-specific methyltransferase; lysine N-methyltransferase 7
Protein Family
Research Articles on SETD7
Similar Products
Product Notes
The SETD7 (Catalog #AAA8105063) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SETD7 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SETD7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the SETD7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SETD7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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