Rabbit anti-Human PDZK1 Polyclonal Antibody | anti-PDZK1 antibody

PDZK1 Antibody (Center)

Cat. Num. AAA9212114

• Gene Names: PDZK1; CAP70; CLAMP; PDZD1; NHERF3; NHERF-3
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PDZK1; Polyclonal Antibody; PDZK1 Antibody (Center); Na(+)/H(+) exchange regulatory cofactor NHE-RF3; NHERF-3; CFTR-associated protein of 70 kDa; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; NaPi-Cap1; PDZ domain-containing protein 1; Sodium-hydrogen exchanger regulatory factor 3; CAP70; NHERF3; PDZD1; anti-PDZK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PDZK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-224 amino acids from the Central region of human PDZK1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.35 (varies by lot)
• Sequence Positions: 196-224
• Sequence Length: 408

• Applicable Applications for anti-PDZK1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(PDZK1 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PDZK1 antibody detected the PDZK1 protein (arrow).)

Immunohistochemistry (IHC)

(PDZK1 Antibody (Center) (MBS9212114)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PDZK1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PDZK1 antibody

A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function (By similarity).

Product Categories/Family for anti-PDZK1 antibody

Metabolism; Signal Transduction

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
LaLonde, D.P., et al. Mol. Biol. Cell 21(9):1519-1529(2010)
Polasek, O., et al. Croat. Med. J. 51(1):32-39(2010)
Gunjaca, G., et al. Croat. Med. J. 51(1):23-31(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 318037203
• NCBI GeneID: 5174
• NCBI Accession #: NP_001188254.1; NP_001188255.1; NP_002605.2
• NCBI GenBank Nucleotide #: NM_001201325.1
• UniProt Accession #: Q5T2W1; O60450; Q5T5P6; Q9BQ41; B4DPB9; E7EU02
• Molecular Weight: 57129
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF3 isoform 1
• NCBI Official Synonym Full Names: PDZ domain containing 1
• NCBI Official Symbol: PDZK1
• NCBI Official Synonym Symbols: CAP70; CLAMP; PDZD1; NHERF3; NHERF-3
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF3
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF3
• Protein Family: PDZK1-interacting protein
• UniProt Gene Name: PDZK1
• UniProt Synonym Gene Names: CAP70; NHERF3; PDZD1; NHERF-3; NaPi-Cap1
• UniProt Entry Name: NHRF3_HUMAN

NCBI Description

This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

PDZK1: A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function. Belongs to the NHER family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: brush border membrane; microvillus membrane; cytoplasm; apical plasma membrane; plasma membrane; lipid raft

Molecular Function: protein binding; scavenger receptor binding; transporter activity; protein complex binding; PDZ domain binding

Biological Process: cell proliferation; drug transport; transport; carnitine transport