Mouse anti-Human SETD7 Monoclonal Antibody | anti-SETD7 antibody

Anti-SETD7 Antibody, Mouse Monoclonal

Cat. Num. AAA8105059

• Gene Names: SETD7; KMT7; SET7; SET9; SET7/9
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A
• Synonyms: SETD7; Monoclonal Antibody; Anti-SETD7 Antibody; Mouse Monoclonal; SETD7/SET7/9 Antibody; Mouse MAb; SET domain containing (lysine methyltransferase) 7; Anti-KMT7 Antibody; Anti-SET7 Antibody; Anti-SET7/9 Antibody; Anti-SET9 Antibody; anti-SETD7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 19
• Specificity: Human SETD7/SET7/9
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SETD7 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Recombinant Human SETD7/SET7/9 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SETD7/SET7/9 (rh SETD7/SET7/9). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-SETD7 mouse monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:41 kDaObserved band size:53 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-SETD7 antibody

Histone-lysine N-methyltransferase SETD7, also known as SET domain containing (lysine methyltransferase) 7, SET7/9, Histone H3-K4 methyltransferase SETD7, H3-K4-HMTase SETD7, and SETD7, is a member of the histone-lysine methyltransferase family and SET7 subfamily. SETD7 is widely expressed and expressed in pancreatic islets. SETD7 contains three MORN repeats and one SET domain. SETD7 plays a central role in the transcriptional activation of genes such as collagenase or insulin. As a protein lysine methyltransferase (PKMT), SETD7 also has methyltransferase activity toward non-histone proteins such as p53/TP53, TAF1, and possibly TAF7 by recognizing and binding in substrate proteins. The mono-methyltransferase activity of SETD7 is achieved by disrupting the formation at near-attack conformations for the dimethylation reaction. SETD7 is also a novel coactivator of NF-kappaB and plays a role in inflammation and diabetes.

References

Wang, H. et al., 2002, Mol Cell 8 (6): 1207-17. Jacobs, SA. et al., 2002, Nat. Struct. Biol. 9 (11): 833-8. Xiao B, et al., 2003, Nature. 421 (6923): 652-6. Martens, JH. et al., 2003, Mol. Cell. Biol. 23: 1808-1816. Couture, JF. et al., 2006, Nat Struct Mol Biol 13 (2): 140-6.

NCBI and Uniprot Product Information

• NCBI GI #: 83035135
• NCBI GeneID: 80854
• NCBI Accession #: NP_085151.1
• NCBI GenBank Nucleotide #: NM_030648
• Molecular Weight: 40,721 Da
• NCBI Official Full Name: Homo sapiens SET domain containing (lysine methyltransferase) 7 (SETD7), mRNA
• NCBI Official Synonym Full Names: SET domain containing (lysine methyltransferase) 7
• NCBI Official Symbol: SETD7
• NCBI Official Synonym Symbols: KMT7; SET7; SET9; SET7/9
• NCBI Protein Information: histone-lysine N-methyltransferase SETD7; H3-K4-HMTase SETD7; SET domain-containing protein 7; histone H3-K4 methyltransferase SETD7; histone H3-lysine 4-specific methyltransferase; lysine N-methyltransferase 7
• Protein Family: Histone-lysine N-methyltransferase