Rabbit anti-Human SETD1A Polyclonal Antibody | anti-SETD1A antibody

SETD1A, ID (SETD1A, KIAA0339, KMT2F, SET1, SET1A, Histone-lysine N-methyltransferase SETD1A, Lysine N-methyltransferase 2F, SET domain-containing protein 1A, Set1/Ash2 histone methyltransferase complex subunit SET1) (Azide free) (HRP)

Cat. Num. AAA6346464

• Gene Names: SETD1A; Set1; KMT2F; Set1A
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SETD1A; Polyclonal Antibody; ID (SETD1A; KIAA0339; KMT2F; SET1; SET1A; Histone-lysine N-methyltransferase SETD1A; Lysine N-methyltransferase 2F; SET domain-containing protein 1A; Set1/Ash2 histone methyltransferase complex subunit SET1) (Azide free) (HRP); Set1/Ash2 histone methyltransferase complex subunit SET1; anti-SETD1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SETD1A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SETD1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 927-956 amino acids from the Central region of human SETD1A.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SETD1A Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SETD1A antibody detected the SETD1A protein (arrow).)

Related Product Information for anti-SETD1A antibody

SET1A is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. The complex is the analog of the S. cerevisiae Set1/COMPASS complex (Lee and Skalnik, 2005 [PubMed 16253997]). Also see SET1B (MIM 611055).

Product Categories/Family for anti-SETD1A antibody

Antibodies; Enzymes; Methyltransferase

References

Wu, M., et al. Mol. Cell. Biol. 28(24):7337-7344(2008). Nguyen, P., et al. Mol. Cell. Biol. 28(21):6720-6729(2008). Ansari, K.I., et al. Biochim. Biophys. Acta 1779(1):66-73(2008). Lee, J.H., et al. Mol. Cell. Biol. 28(2):609-618(2008). Cho, Y.W., et al. J. Biol. Chem. 282(28):20395-20406(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 55741677
• NCBI GeneID: 9739
• NCBI Accession #: NP_055527.1
• NCBI GenBank Nucleotide #: NM_014712.1
• UniProt Accession #: O15047; Q6PIF3; Q8TAJ6; A6NP62
• Molecular Weight: 186,034 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SETD1A
• NCBI Official Synonym Full Names: SET domain containing 1A
• NCBI Official Symbol: SETD1A
• NCBI Official Synonym Symbols: Set1; KMT2F; Set1A
• NCBI Protein Information: histone-lysine N-methyltransferase SETD1A; hSET1A; lysine N-methyltransferase 2F; SET domain-containing protein 1A; set1/Ash2 histone methyltransferase complex subunit SET1
• UniProt Protein Name: Histone-lysine N-methyltransferase SETD1A
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SETD1A
• UniProt Synonym Gene Names: hSET1A

Uniprot Description

SETD1A: Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys- 9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.

Protein type: Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: histone methyltransferase complex; nuclear chromatin; nuclear speck; nucleoplasm; nucleus

Molecular Function: beta-catenin binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; protein binding; transcription factor binding