Western Blot (WB)
(SETD1A Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SETD1A antibody detected the SETD1A protein (arrow).)
Rabbit anti-Human SETD1A Polyclonal Antibody | anti-SETD1A antibody
SETD1A, ID (SETD1A, KIAA0339, KMT2F, SET1, SET1A, Histone-lysine N-methyltransferase SETD1A, Lysine N-methyltransferase 2F, SET domain-containing protein 1A, Set1/Ash2 histone methyltransferase complex subunit SET1) (PE)
Gene Names
SETD1A; Set1; KMT2F; Set1A
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SETD1A; Polyclonal Antibody; ID (SETD1A; KIAA0339; KMT2F; SET1; SET1A; Histone-lysine N-methyltransferase SETD1A; Lysine N-methyltransferase 2F; SET domain-containing protein 1A; Set1/Ash2 histone methyltransferase complex subunit SET1) (PE); Set1/Ash2 histone methyltransferase complex subunit SET1; anti-SETD1A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-SETD1A antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
SETD1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 927-956 amino acids from the Central region of human SETD1A.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(SETD1A Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SETD1A antibody detected the SETD1A protein (arrow).)
Western Blot (WB)
(SETD1A Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SETD1A antibody detected the SETD1A protein (arrow).)
Related Product Information for anti-SETD1A antibody
SET1A is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. The complex is the analog of the S. cerevisiae Set1/COMPASS complex (Lee and Skalnik, 2005 [PubMed 16253997]). Also see SET1B (MIM 611055).
Product Categories/Family for anti-SETD1A antibody
References
Wu, M., et al. Mol. Cell. Biol. 28(24):7337-7344(2008). Nguyen, P., et al. Mol. Cell. Biol. 28(21):6720-6729(2008). Ansari, K.I., et al. Biochim. Biophys. Acta 1779(1):66-73(2008). Lee, J.H., et al. Mol. Cell. Biol. 28(2):609-618(2008). Cho, Y.W., et al. J. Biol. Chem. 282(28):20395-20406(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
186,034 Da
NCBI Official Full Name
histone-lysine N-methyltransferase SETD1A
NCBI Official Synonym Full Names
SET domain containing 1A
NCBI Official Symbol
SETD1A
NCBI Official Synonym Symbols
Set1; KMT2F; Set1A
NCBI Protein Information
histone-lysine N-methyltransferase SETD1A; hSET1A; lysine N-methyltransferase 2F; SET domain-containing protein 1A; set1/Ash2 histone methyltransferase complex subunit SET1
UniProt Protein Name
Histone-lysine N-methyltransferase SETD1A
Protein Family
UniProt Gene Name
SETD1A
UniProt Synonym Gene Names
; hSET1A
Uniprot Description
SETD1A: Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys- 9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
Protein type: Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: histone methyltransferase complex; nuclear chromatin; nuclear speck; nucleoplasm; nucleus
Molecular Function: beta-catenin binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; protein binding; transcription factor binding
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Product Notes
The SETD1A setd1a (Catalog #AAA6346470) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SETD1A, ID (SETD1A, KIAA0339, KMT2F, SET1, SET1A, Histone-lysine N-methyltransferase SETD1A, Lysine N-methyltransferase 2F, SET domain-containing protein 1A, Set1/Ash2 histone methyltransferase complex subunit SET1) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SETD1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SETD1A setd1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SETD1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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