Rabbit anti-Human, Mouse Sestrin-1 Polyclonal Antibody | anti-SEST1 antibody

Sestrin-1, NT (SESN1, PA26, SEST1, Sestrin-1, p53-regulated protein PA26) (MaxLight 490)

Cat. Num. AAA6346422

• Gene Names: SESN1; PA26; SEST1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: Sestrin-1; Polyclonal Antibody; NT (SESN1; PA26; SEST1; p53-regulated protein PA26) (MaxLight 490); SESN1; p53-regulated protein PA26; anti-SEST1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Sestrin-1. Species Crossreactivity: mouse
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-SEST1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Sestrin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21~51 amino acids from the N-terminal region of human Sestrin-1.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Sestrin-1 Antibody (N-term) in mouse heart tissue lysates (35ug/lane). Sestrin-1 (arrow) was detected using the purified Pab.)

Related Product Information for anti-SEST1 antibody

Sestrin-1 is involved in the reduction of peroxiredoxins. This protein may also be regulator of cellular growth.

Product Categories/Family for anti-SEST1 antibody

Antibodies; Proteins

References

Budanov, A.V., Science 304 (5670), 596-600 (2004). Peeters, H., Hum. Genet. 112 (5-6), 573-580 (2003). Velasco-Miguel, S., Oncogene 18 (1), 127-137 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 315709500
• NCBI GeneID: 27244
• NCBI Accession #: NP_001186862.1
• NCBI GenBank Nucleotide #: NM_001199933.1
• UniProt Accession #: Q9Y6P5; Q2M2B7; Q5T316; Q9NV00; Q9UPD5; Q9Y6P6
• Molecular Weight: 56,557 Da
• NCBI Official Full Name: sestrin-1 isoform 2
• NCBI Official Synonym Full Names: sestrin 1
• NCBI Official Symbol: SESN1
• NCBI Official Synonym Symbols: PA26; SEST1
• NCBI Protein Information: sestrin-1; p53 activated gene 26; p53 regulated PA26 nuclear protein
• UniProt Protein Name: Sestrin-1
• Protein Family: Sestrin
• UniProt Gene Name: SESN1
• UniProt Synonym Gene Names: PA26; SEST1
• UniProt Entry Name: SESN1_HUMAN

NCBI Description

This gene encodes a member of the sestrin family. Sestrins are induced by the p53 tumor suppressor protein and play a role in the cellular response to DNA damage and oxidative stress. The encoded protein mediates p53 inhibition of cell growth by activating AMP-activated protein kinase, which results in the inhibition of the mammalian target of rapamycin protein. The encoded protein also plays a critical role in antioxidant defense by regenerating overoxidized peroxiredoxins, and the expression of this gene is a potential marker for exposure to radiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

SESN1: Involved in the reduction of peroxiredoxins. May also be regulator of cellular growth. Belongs to the sestrin family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleus

Biological Process: negative regulation of cell proliferation; regulation of protein kinase B signaling cascade; cell cycle arrest; response to DNA damage stimulus