Western Blot (WB)
(Western Blot analysis of ZNF74 expression in transfected 293T cell line by ZNF74 polyclonal antibody. Lane 1: ZNF74 transfected lysate (70.84kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human ZNF74 Polyclonal Antibody | anti-ZNF74 antibody
ZNF74 (Zinc Finger Protein 74, COS52, hZNF7, Zinc Finger Protein 520, ZFP520, ZNF520)
Gene Names
ZNF74; COS52; hZNF7; ZFP520; ZNF520
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
ZNF74; Polyclonal Antibody; ZNF74 (Zinc Finger Protein 74; COS52; hZNF7; Zinc Finger Protein 520; ZFP520; ZNF520); Anti -ZNF74 (Zinc Finger Protein 74; anti-ZNF74 antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ZNF74.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MEIPAPEPEKTALSSQDPALSLKENLEDISGWGLPEARSKESVSFKDVAVDFTQEEWGQLDSPQRALYRDVMLENYQNLLALGPPLHKPDVISHLERGEEPWSMQREVPRGPCPEWELKAVPSQQQGICKEEPAQEPIMERPLGGAQAWGRQAGALQRSQAAPWAPAPAMAWDVPVEEFPLRCPLFAQQRVPEGGPLLDTRKNVQATEGRTKAPARLCAGENASTPSEPEKFPQVRRQRGAGAGEGEFVCGECGKAFRQSSSLTLHRRWHSREKAYKCDECGKAFTWSTNLLEHRRIHTGEKPFFCGECGKAFSCHSSLNVHQRIHTGERPYKCSACEKAFSCSSLLSMHLRVHTGEKPYRCGECGKAFNQRTHLTRHHRIHTGEKPYQCGSCGKAFTCHSSLTVHEKIHSGDKPFKCSDCEKAFNSRSRLTLHQRTHTGEKPFKCADCGKGFSCHAYLLVHRRIHSGEKPFKCNECGKAFSSHAYLIVHRRIHTGEKPFDCSQCWKAFSCHSSLIVHQRIHTGEKPYKCSECGRAFSQNHCLIKHQKIHSGEKSFKCEKCGEMFNWSSHLTEHQRLHSEGKPLAIQFNKHLLSTYYVPGSLLGAGDAGLRDVDPIDALDVAKLLCVVPPRAGRNFSLGSKPRN
Applicable Applications for anti-ZNF74 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot and Immunofluorescence.
Dilution: Immunofluorescence: 10ug/ml
Dilution: Immunofluorescence: 10ug/ml
Immunogen
Full length human ZNF74, aa1-644 (AAH13395.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of ZNF74 expression in transfected 293T cell line by ZNF74 polyclonal antibody. Lane 1: ZNF74 transfected lysate (70.84kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of ZNF74 expression in transfected 293T cell line by ZNF74 polyclonal antibody. Lane 1: ZNF74 transfected lysate (70.84kD). Lane 2: Non-transfected lysate.)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to ZNF74 on HeLa cell. [antibody concentration 10ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to ZNF74 on HeLa cell. [antibody concentration 10ug/ml])
Product Categories/Family for anti-ZNF74 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,207 Da
NCBI Official Full Name
zinc finger protein 74 isoform c
NCBI Official Synonym Full Names
zinc finger protein 74
NCBI Official Symbol
ZNF74
NCBI Official Synonym Symbols
COS52; hZNF7; ZFP520; ZNF520
NCBI Protein Information
zinc finger protein 74; zinc finger protein 520
UniProt Protein Name
Zinc finger protein 74
Protein Family
UniProt Gene Name
ZNF74
UniProt Synonym Gene Names
ZNF520
UniProt Entry Name
ZNF74_HUMAN
Uniprot Description
Function: May play a role in RNA metabolism.
Subcellular location: Nucleus.
Tissue specificity: Highly expressed in the fetal brain.
Sequence similarities: Belongs to the krueppel C2H2-type zinc-finger protein family.Contains 12 C2H2-type zinc fingers.Contains 1 KRAB domain.
Sequence caution: The sequence AAF21777.1 differs from that shown. Reason: Frameshift at positions 164 and 173. The sequence AAF21778.1 differs from that shown. Reason: Frameshift at positions 164 and 173. The sequence AAF21779.1 differs from that shown. Reason: Frameshift at positions 164 and 173. The sequence AAF21780.1 differs from that shown. Reason: Frameshift at positions 164 and 173. The sequence CAA50632.1 differs from that shown. Reason: Frameshift at positions 164 and 173. The sequence CAA63379.1 differs from that shown. Reason: Frameshift at positions 164 and 173.
Research Articles on ZNF74
Similar Products
Product Notes
The ZNF74 znf74 (Catalog #AAA647622) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ZNF74 (Zinc Finger Protein 74, COS52, hZNF7, Zinc Finger Protein 520, ZFP520, ZNF520) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF74 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Western Blot and Immunofluorescence. Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the ZNF74 znf74 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MEIPAPEPEK TALSSQDPAL SLKENLEDIS GWGLPEARSK ESVSFKDVAV DFTQEEWGQL DSPQRALYRD VMLENYQNLL ALGPPLHKPD VISHLERGEE PWSMQREVPR GPCPEWELKA VPSQQQGICK EEPAQEPIME RPLGGAQAWG RQAGALQRSQ AAPWAPAPAM AWDVPVEEFP LRCPLFAQQR VPEGGPLLDT RKNVQATEGR TKAPARLCAG ENASTPSEPE KFPQVRRQRG AGAGEGEFVC GECGKAFRQS SSLTLHRRWH SREKAYKCDE CGKAFTWSTN LLEHRRIHTG EKPFFCGECG KAFSCHSSLN VHQRIHTGER PYKCSACEKA FSCSSLLSMH LRVHTGEKPY RCGECGKAFN QRTHLTRHHR IHTGEKPYQC GSCGKAFTCH SSLTVHEKIH SGDKPFKCSD CEKAFNSRSR LTLHQRTHTG EKPFKCADCG KGFSCHAYLL VHRRIHSGEK PFKCNECGKA FSSHAYLIVH RRIHTGEKPF DCSQCWKAFS CHSSLIVHQR IHTGEKPYKC SECGRAFSQN HCLIKHQKIH SGEKSFKCEK CGEMFNWSSH LTEHQRLHSE GKPLAIQFNK HLLSTYYVPG SLLGAGDAGL RDVDPIDALD VAKLLCVVPP RAGRNFSLGS KPRN. It is sometimes possible for the material contained within the vial of "ZNF74, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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