Immunohistochemistry (IHC)
(MBS9607633 at 1/100 staining Human cervical cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit SERPINH1 Polyclonal Antibody | anti-SERPINH1 antibody
SERPINH1 Antibody
Gene Names
SERPINH1; CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SERPINH1; Polyclonal Antibody; SERPINH1 Antibody; 47 kDa heat shock protein; 47 kDa heat shock protein precursor; Arsenic transactivated protein 3; Arsenic-transactivated protein 3; AsTP 3; AsTP3; CBP 1; CBP 2; CBP1; CBP2; Cell proliferation-inducing gene 14 protein; Collagen binding protein 1; Collagen binding protein 2; Collagen binding protein; Collagen-binding protein 2; Collagen-binding protein; Colligen; Colligin 1; Colligin 2; Colligin; colligin-1; colligin-2; gp46; Heat shock protein 47; Heat-shock protein 47; HGNC 1547; Hsp 47; J6; OI10; PIG 14; PIG14; PPROM; Proliferation inducing gene 14; Proliferation inducing gene 14 protein; RA A47; RA-A47; Rheumatoid arthritis antigen A 47; rheumatoid arthritis antigen A-47; Rheumatoid arthritis related antigen RA A47; Rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor; clade H (heat shock protein 47); member 1; (collagen binding protein 1); member 2; (collagen-binding protein 2); Serine or cysteine proteinase inhibitor clade H member 1; Serine or cysteine proteinase inhibitor clade H member 2; SERPH_HUMAN; Serpin H1; Serpin peptidase inhibitor clade H member 1; serpin peptidase inhibitor; Serpin peptidase inhibitor; clade H; SERPINH2; anti-SERPINH1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SERPINH1 antibody detects endogenous levels of total SERPINH1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
418
Applicable Applications for anti-SERPINH1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human SERPINH1
Subcellular Location
Endoplasmic Reticulum Lumen.
Tissue Specificity
By heat shock.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9607633 at 1/100 staining Human cervical cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9607633 at 1/100 staining Human cervical cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of HepG2 cell lysate, using SERPINH1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of HepG2 cell lysate, using SERPINH1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-SERPINH1 antibody
Description: This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9.
Function: Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Similarity: Belongs to the serpin family.
Function: Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Similarity: Belongs to the serpin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 46 kDa
Predicted: 47 kDa
Predicted: 47 kDa
NCBI Official Full Name
serpin H1
NCBI Official Synonym Full Names
serpin family H member 1
NCBI Official Symbol
SERPINH1
NCBI Official Synonym Symbols
CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
NCBI Protein Information
serpin H1
UniProt Protein Name
Serpin H1
Protein Family
UniProt Gene Name
SERPINH1
UniProt Synonym Gene Names
CBP1; CBP2; HSP47; SERPINH2; AsTP3; Colligin
NCBI Description
This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]
Uniprot Description
Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Research Articles on SERPINH1
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Product Notes
The SERPINH1 serpinh1 (Catalog #AAA9607633) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SERPINH1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SERPINH1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the SERPINH1 serpinh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SERPINH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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