Western Blot (WB)
(Western Blot (WB) analysis of SERPINB3/4 polyclonal antibody at 1:500 dilution Line1:THP-1 whole cell lysateLine2:PC12 whole cell lysateLine3:sp20 whole cell lysate)
Rabbit SERPINB3/4 Polyclonal Antibody | anti-SERPINB3/4 antibody
SERPINB3/4 Polyclonal Antibody
Gene Names
SERPINB3; SCC; T4-A; SCCA1; SSCA1; SCCA-1; HsT1196; SCCA-PD
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SERPINB3/4; Polyclonal Antibody; SERPINB3/4 Polyclonal Antibody; Serpin B3; Protein T4-A; Squamous cell carcinoma antigen 1; SCCA-1; SERPINB3; SCCA; SCCA1; Serpin B4; Leupin; Peptidase inhibitor 11; PI-11; Squamous cell carcinoma antigen 2; SCCA-2; SERPINB4; PI11; SCCA2; anti-SERPINB3/4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SERPINB3/4 polyclonal antibody detects endogenous levels of SERPINB3/4 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
390
Applicable Applications for anti-SERPINB3/4 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human SERPINB3/4.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of SERPINB3/4 polyclonal antibody at 1:500 dilution Line1:THP-1 whole cell lysateLine2:PC12 whole cell lysateLine3:sp20 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of SERPINB3/4 polyclonal antibody at 1:500 dilution Line1:THP-1 whole cell lysateLine2:PC12 whole cell lysateLine3:sp20 whole cell lysate)
Related Product Information for anti-SERPINB3/4 antibody
Metastasis of a primary tumor to a distant site is determined through signaling cascades that break down interactions between the cell and extracellular matrix proteins. Among the proteins mediating metastasis are serine prote-ases, such as neutrophil elastase. In 1985, Dr. Jim Travis and Dr. R.W. Carrell designated an emerging family of serine protease inhibitors as the serpin fam-ily, which share homology in both primary amino acid sequence and tertiary structure. Serpins contain a stretch of peptide that mimics a true substrate for a corresponding serine protease. Serine proteases bind to this substrate mimic in a 1: 1 stoichiometric fashion and become catalytically inactive. Aberrant ex-pression of serpin family members can contribute to a number of conditions, including emphysema (a-1 antitrypsin deficiency), fatal bleeding (elastase to thrombin specificity) and thrombosis (antithrombin deficiency), and are indicators of cancer stage phenotypes (circulating levels of squamous cell carcinoma antigen, known as SCCA1, increase in advancing stages of some cervical, lung, esophageal and head and neck cancers). Human chromosome position 18q21.3 contains a cluster of serpins, including a tandem duplication of the SCCA gene, plasminogen activator inhibitor type 2, and maspin. SCCA is transcribed by two nearly identical genes (SCCA1 and SCCA2), and is mainly produced as SCCA1. The human SCCA1 gene encodes a 390 amino acid protein that was originally isolated from a metastatic cervical squamous cell carcinoma.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~ 44kDa
NCBI Official Full Name
serpin B3
NCBI Official Synonym Full Names
serpin family B member 3
NCBI Official Symbol
SERPINB3
NCBI Official Synonym Symbols
SCC; T4-A; SCCA1; SSCA1; SCCA-1; HsT1196; SCCA-PD
NCBI Protein Information
serpin B3
UniProt Protein Name
Serpin B3
UniProt Gene Name
SERPINB3
UniProt Synonym Gene Names
SCCA; SCCA1; SCCA-1
UniProt Entry Name
SPB3_HUMAN
Uniprot Description
SERPINB3: May act as a protease inhibitor to modulate the host immune response against tumor cells. Belongs to the serpin family. Ov-serpin subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 18q21.3
Cellular Component: cytoplasm; cytoplasmic vesicle; extracellular space; nucleus; vesicle
Molecular Function: cysteine protease inhibitor activity; protease binding; serine-type endopeptidase inhibitor activity; viral receptor activity
Biological Process: negative regulation of catalytic activity; negative regulation of JNK activity; negative regulation of peptidase activity; negative regulation of proteolysis; positive regulation of cell migration; positive regulation of cell proliferation
Research Articles on SERPINB3/4
Similar Products
Product Notes
The SERPINB3/4 serpinb3 (Catalog #AAA3007170) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SERPINB3/4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SERPINB3/4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the SERPINB3/4 serpinb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SERPINB3/4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
