Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Immunoprecipitation (IP) (SERPINB3 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-SERPINB3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SERPINB3 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 42 kDa)

Rabbit anti-Human SerpinB3 Monoclonal Antibody | anti-SERPINB3 antibody

Recombinant Anti-SerpinB3 Antibody, Rabbit Monoclonal

Gene Names
SERPINB3; SCC; T4-A; SCCA1; SSCA1; SCCA-1; HsT1196; SCCA-PD
Reactivity
Human
Applications
Immunoprecipitation
Purity
Protein A
Synonyms
SerpinB3; Monoclonal Antibody; Recombinant Anti-SerpinB3 Antibody; Rabbit Monoclonal; SERPINB3/SCCA-1 Antibody; Rabbit MAb; serpin peptidase inhibitor; clade B (ovalbumin); member 3; Anti-HsT1196 Antibody; Anti-SCC Antibody; Anti-SCCA-1 Antibody; Anti-SCCA-PD Antibody; Anti-SCCA1 Antibody; Anti-T4-A Antibody; anti-SERPINB3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
018
Specificity
Human SERPINB3/SCCA-1
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SERPINB3 antibody
Immunoprecipitation (IP)
Application Notes
IP: 4-8uL/mg of lysate
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human SERPINB3/SCCA-1 Protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human SERPINB3/SCCA-1 (rh SERPINB3/SCCA-1).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(SERPINB3 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-SERPINB3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SERPINB3 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 42 kDa)

Immunoprecipitation (IP) (SERPINB3 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-SERPINB3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SERPINB3 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 42 kDa)
Related Product Information for anti-SERPINB3 antibody
SERPINB3, also known as SCCA-1, belongs to the serpin family. Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases. SERPINB3 is expressed in some hepatocellular carcinoma (at protein level). Its expression is closely related to cellular differentiation in both normal and malignant squamous cells. It seems to also be secreted in plasma by cancerous cells but at a low level. SERPINB3 significantly attenuates apoptosis by contrasting cytochrome c release from the mitochondria and by antichemotactic effect for NK cells. It may act as a protease inhibitor to modulate the host immune response against tumor cells and may be involved in the malignant behavior of squamous cell carcinoma cells.
Product Categories/Family for anti-SERPINB3 antibody
References
Schneider SS, et al. (1995) A serine proteinase inhibitor locus at 18q21.3 contains a tandem duplication of the human squamous cell carcinoma antigen gene. Proc Natl Acad Sci. 92(8):3147-51.Suminami Y, et al.. (1992) Squamous cell carcinoma antigen is a new member of the serine protease inhibitors. Biochem Biophys Res Commun. 181(1):51-8.Mino-Miyagawa N, et al. (1990) Tumor-antigen 4. Its immunohistochemical distribution and tissue and serum concentrations in squamous cell carcinoma of the lung and esophagus. Cancer. 66(7):1505-12.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
38,550 Da
NCBI Official Full Name
squamous cell carcinoma antigen
NCBI Official Synonym Full Names
serpin family B member 3
NCBI Official Symbol
SERPINB3
NCBI Official Synonym Symbols
SCC; T4-A; SCCA1; SSCA1; SCCA-1; HsT1196; SCCA-PD
NCBI Protein Information
serpin B3
UniProt Protein Name
Serpin B3
Protein Family
UniProt Gene Name
SERPINB3
UniProt Synonym Gene Names
SCCA; SCCA1; SCCA-1
UniProt Entry Name
SPB3_HUMAN

Uniprot Description

SERPINB3: May act as a protease inhibitor to modulate the host immune response against tumor cells. Belongs to the serpin family. Ov-serpin subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q21.3

Cellular Component: cytoplasm; cytoplasmic vesicle; extracellular space; nucleus; vesicle

Molecular Function: cysteine protease inhibitor activity; protease binding; serine-type endopeptidase inhibitor activity; viral receptor activity

Biological Process: negative regulation of catalytic activity; negative regulation of JNK activity; negative regulation of peptidase activity; negative regulation of proteolysis; positive regulation of cell migration; positive regulation of cell proliferation

Research Articles on SERPINB3

Similar Products

Product Notes

The SERPINB3 serpinb3 (Catalog #AAA8106841) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-SerpinB3 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SerpinB3 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). IP: 4-8uL/mg of lysate. Researchers should empirically determine the suitability of the SERPINB3 serpinb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SerpinB3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.