Immunohistochemistry (IHC)
(Anti-SERPINB1 antibody IHC staining of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Goat anti-Human SERPINB1 Polyclonal Antibody | anti-SERPINB1 antibody
Anti-SERPINB1 Antibody (aa65-80)
Gene Names
SERPINB1; EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2; HEL-S-27
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
SERPINB1; Polyclonal Antibody; Anti-SERPINB1 Antibody (aa65-80); Mouse Monoclonal [clone 3B4] (IgG2b) to Human SERPINB1; Anti-elastase; EI; LEI; Leukocyte elastase inhibitor; Peptidase inhibitor 2; PI-2; MNEI; ELANH2; M/NEI; PI2; Serpin B1; anti-SERPINB1 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Specificity
Human SERPINB1
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Sequence Length
379
Applicable Applications for anti-SERPINB1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
ELISA: 1:128000
IHC-P: 5 ug/ml
WB: 1-3 ug/ml
IHC-P: 5 ug/ml
WB: 1-3 ug/ml
Immunogen
Synthetic peptide C-EVHSRFQSLNADINKR from an internal region of human SERPINB1 (NP_109591.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Platypus (100%); Ferret, Panda (94%); Bat, Elephant (88%); Monkey, Sheep, Bovine, Horse, Pig (81%); Rat (75%); Mouse (69%).
Conjugation
Unconjugated
Epitope
aa65-80
Preparation and Storage
Store at -20 degree C.
Avoid freeze-thaw cycles.
Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Anti-SERPINB1 antibody IHC staining of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-SERPINB1 antibody IHC staining of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB)
(SERPINB1 antibody (1 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(SERPINB1 antibody (1 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Related Product Information for anti-SERPINB1 antibody
SERPINB1 is a member of the serpin family of protein ase inhibitors.Members of this family maintain homeostasis by neutralizingoverexpressed protein ase activity through their function as suicidesubstrates. This protein inhibits the neutrophil-derived protein asesneutrophil elastase, cathepsin G, and protein ase-3 and thus protectstissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,073 Da
NCBI Official Full Name
leukocyte elastase inhibitor
NCBI Official Synonym Full Names
serpin family B member 1
NCBI Official Symbol
SERPINB1
NCBI Official Synonym Symbols
EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2; HEL-S-27
NCBI Protein Information
leukocyte elastase inhibitor
UniProt Protein Name
Leukocyte elastase inhibitor
Protein Family
UniProt Gene Name
SERPINB1
UniProt Synonym Gene Names
ELANH2; MNEI; PI2; LEI; EI; M/NEI; PI-2
NCBI Description
The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Uniprot Description
Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Also functions as a potent intracellular inhibitor of granzyme H.
Research Articles on SERPINB1
Similar Products
Product Notes
The SERPINB1 serpinb1 (Catalog #AAA2401056) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-SERPINB1 Antibody (aa65-80) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SERPINB1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ELISA: 1:128000 IHC-P: 5 ug/ml WB: 1-3 ug/ml. Researchers should empirically determine the suitability of the SERPINB1 serpinb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SERPINB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
