Western Blot (WB)
(Western blot analysis of SERPINA1 expression in rat brain extract (lane 1) and HELA whole cell lysates (lane 2). SERPINA1 at 52KD was detected using rabbit anti- SERPINA1 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rabbit anti-Human, Rat SERPINA1 Polyclonal Antibody | anti-SERPINA1 antibody
Anti-SERPINA1 Antibody
Gene Names
SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
Reactivity
Human, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
SERPINA1; Polyclonal Antibody; Anti-SERPINA1 Antibody; A1AT; Alpha 1 antiproteinase; Alpha 1 antitrypsin null; alpha1 antitrypsin; alpha1 PI; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Alpha-1-antitrypsin; Alpha-1-proteinase inhibitor; Serpin A1; SERPINA 1; Short peptide from AAT; SPAAT; P01009; serpin peptidase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 1; anti-SERPINA1 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
418
Applicable Applications for anti-SERPINA1 antibody
Western Blot (WB)
Application Notes
Western Blot: 0.1-0.5ug/ml
Notes
Tested Species: In-house tested species with positive results.
Other applications have not been tested.
Other applications have not been tested.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E. coli-derived human SERPINA1 recombinant protein (Position: E25-T204). Human SERPINA1 shares 72.8% amino acid (aa) sequence identity with rat SERPINA1.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Western blot analysis of SERPINA1 expression in rat brain extract (lane 1) and HELA whole cell lysates (lane 2). SERPINA1 at 52KD was detected using rabbit anti- SERPINA1 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of SERPINA1 expression in rat brain extract (lane 1) and HELA whole cell lysates (lane 2). SERPINA1 at 52KD was detected using rabbit anti- SERPINA1 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Related Product Information for anti-SERPINA1 antibody
Rabbit IgG polyclonal antibody for Alpha-1-antitrypsin(SERPINA1) detection.
Background: SERPINA1 is also known as PI, A1A or AAT. This gene is mapped to 14q32.1. The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
Background: SERPINA1 is also known as PI, A1A or AAT. This gene is mapped to 14q32.1. The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
References
1. Alpha 1-antitrypsin therapy mitigated ischemic stroke damage in rats.Moldthan HL, et al. J Stroke Cerebrovasc Dis, 2014 May-Jun.
2. Mixture-based combinatorial libraries from small individual peptide libraries: a case study on 1-antitrypsin deficiency.Chang YP, et al. Molecules, 2014 May 16.
3. Serpin peptidase inhibitor clade A member 1 is a biomarker of poor prognosis in gastric cancer.Kwon CH, et al. Br J Cancer, 2014 Nov 11.
2. Mixture-based combinatorial libraries from small individual peptide libraries: a case study on 1-antitrypsin deficiency.Chang YP, et al. Molecules, 2014 May 16.
3. Serpin peptidase inhibitor clade A member 1 is a biomarker of poor prognosis in gastric cancer.Kwon CH, et al. Br J Cancer, 2014 Nov 11.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,755 Da
NCBI Official Full Name
alpha-1-antitrypsin
NCBI Official Synonym Full Names
serpin family A member 1
NCBI Official Symbol
SERPINA1
NCBI Official Synonym Symbols
PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
NCBI Protein Information
alpha-1-antitrypsin
UniProt Protein Name
Alpha-1-antitrypsin
Protein Family
UniProt Gene Name
SERPINA1
UniProt Synonym Gene Names
AAT; PI; SPAAT
NCBI Description
The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Research Articles on SERPINA1
Similar Products
Product Notes
The SERPINA1 serpina1 (Catalog #AAA178853) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SERPINA1 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SERPINA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the SERPINA1 serpina1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SERPINA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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