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ELISA (EIA) (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CD30 protein, His tagged protein can bind Rabbit anti-CD30 monoclonal antibody (clone: DM105) in a linear range of 0.12-80 ng/ml.)

Rabbit anti-Human CD30 Monoclonal Antibody | anti-CD30 antibody

Anti-CD30 antibody (DM105), Rabbit mAb

Gene Names
TNFRSF8; CD30; Ki-1; D1S166E
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Purified from cell culture supernatant by affinity chromatography
Synonyms
CD30; Monoclonal Antibody; Anti-CD30 antibody (DM105); Rabbit mAb; TNFRSF8; D1S166E; Ki-1; anti-CD30 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
DM105
Purity/Purification
Purified from cell culture supernatant by affinity chromatography
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose is added as protectants before lyophilization. 0.1% Procline 300
Applicable Applications for anti-CD30 antibody
ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
ELISA: 1:5000-10,000
FC/FACS: 1:100
Immunogen
Recombinant human CD30 (Phe19-Lys379) produced by using human HEK293 cells
Reconstitution
Reconstitute with deionized water
Preparation and Storage
Store at -20 degree C for 12 months (Avoid repeated freezing and thawing)

ELISA (EIA)

(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CD30 protein, His tagged protein can bind Rabbit anti-CD30 monoclonal antibody (clone: DM105) in a linear range of 0.12-80 ng/ml.)

ELISA (EIA) (Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CD30 protein, His tagged protein can bind Rabbit anti-CD30 monoclonal antibody (clone: DM105) in a linear range of 0.12-80 ng/ml.)

Flow Cytometry (FC/FACS)

(Figure 2. Flow cytometry analysis with Anti-CD30 (DM105) on Expi293 cells transfected with human CD30 (Blue histogram)or Expi293 transfected with irrelevant protein (Red histogram).)

Flow Cytometry (FC/FACS) (Figure 2. Flow cytometry analysis with Anti-CD30 (DM105) on Expi293 cells transfected with human CD30 (Blue histogram)or Expi293 transfected with irrelevant protein (Red histogram).)
Related Product Information for anti-CD30 antibody
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
943
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,935 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 8 isoform 1
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily member 8
NCBI Official Symbol
TNFRSF8
NCBI Official Synonym Symbols
CD30; Ki-1; D1S166E
NCBI Protein Information
tumor necrosis factor receptor superfamily member 8
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 8
Protein Family
UniProt Gene Name
TNFRSF8
UniProt Synonym Gene Names
CD30; D1S166E
UniProt Entry Name
TNR8_HUMAN

Uniprot Description

TNFRSF8: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: integral to plasma membrane

Molecular Function: transmembrane receptor activity; tumor necrosis factor receptor activity

Biological Process: immune response; inflammatory response; multicellular organismal development; negative regulation of cell proliferation; positive regulation of apoptosis; positive regulation of TRAIL biosynthetic process; positive regulation of tumor necrosis factor biosynthetic process; response to lipopolysaccharide; signal transduction

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Product Notes

The CD30 tnfrsf8 (Catalog #AAA188630) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD30 antibody (DM105), Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD30 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:5000-10,000 FC/FACS: 1:100. Researchers should empirically determine the suitability of the CD30 tnfrsf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD30, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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