Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: A172 cells, Primary antibody: 162179(SEPN1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes)
Rabbit anti-Human SEPN1/SELN Polyclonal Antibody | anti-SEPN1/SELN antibody
SEPN1/SELN Rabbit pAb
IHC: 1:25-1:100
Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: A172 cells, Primary antibody: 162179(SEPN1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes)
Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: A172 cells, Primary antibody: 162179(SEPN1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes)
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 162179(SEPN1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 162179(SEPN1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human brain tissue using 162179(SEPN1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human brain tissue using 162179(SEPN1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a glycoprotein that is localized in the endoplasmic reticulum. It plays an important role in cell protection against oxidative stress, and in the regulation of redox-related calcium homeostasis. Mutations in this gene are associated with early onset muscle disorders, referred to as SEPN1-related myopathy. SEPN1-related myopathy consists of 4 autosomal recessive disorders, originally thought to be separate entities: rigid spine muscular dystrophy (RSMD1), the classical form of multiminicore disease, desmin related myopathy with Mallory-body like inclusions, and congenital fiber-type disproportion (CFTD). This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A second stop-codon redefinition element (SRE) adjacent to the UGA codon has been identified in this gene (PMID:15791204). SRE is a phylogenetically conserved stem-loop structure that stimulates readthrough at the UGA codon, and augments the Sec insertion efficiency by SECIS. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2016]
Uniprot Description
Subcellular location: Isoform 2: Endoplasmic reticulum membrane
Probable. Note: Probably membrane-associated. Ref.5
Tissue specificity: Isoform 1 and isoform 2 are expressed in skeletal muscle, brain, lung and placenta. Isoform 2 is also expressed in heart, diaphragm and stomach. Ref.1 Ref.5
Domain: The N-terminus (first 61 amino acids) contains an endoplasmic reticulum addressing and retention targeting signal.
Post-translational modification: N-glycosylated (isoform 2).
Involvement in disease: Rigid spine muscular dystrophy 1 (RSMD1) [MIM:602771]: A neuromuscular disorder characterized by poor axial muscle strength, scoliosis and neck weakness, and a variable degree of spinal rigidity. Early ventilatory insufficiency can lead to death by respiratory failure.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.1 Ref.6 Ref.7 Ref.8
Sequence similarities: Contains 1 EF-hand domain.
Sequence caution: The sequence AAH15638.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH42154.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on SEPN1/SELN
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Product Notes
The SEPN1/SELN sepn1 (Catalog #AAA4754880) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SEPN1/SELN Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SEPN1/SELN can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC: 1:25-1:100. Researchers should empirically determine the suitability of the SEPN1/SELN sepn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEPN1/SELN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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