Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (All lanes: FBXW4 antibody at 4ug/ml+mouse skeletal muscle tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 46kDaObserved band size: 46kDa)

Rabbit anti-Human, Mouse FBXW4 Polyclonal Antibody | anti-FBXW4 antibody

Rabbit anti-human FBXW4 polyclonal Antibody

Gene Names
FBXW4; DAC; FBW4; FBWD4; SHFM3; SHSF3
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Synonyms
FBXW4; Polyclonal Antibody; Rabbit anti-human FBXW4 polyclonal Antibody; Dactylin; F-box and WD-40 domain-containing protein 4; FBW4; SHFM3; anti-FBXW4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
412
Applicable Applications for anti-FBXW4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000
IHC:1:20-1:200
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human F-box/WD repeat-containing protein 4 protein (143-412aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-163369 / sc-163370 / sc-130266 / sc-292027
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: FBXW4 antibody at 4ug/ml+mouse skeletal muscle tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 46kDaObserved band size: 46kDa)

Western Blot (WB) (All lanes: FBXW4 antibody at 4ug/ml+mouse skeletal muscle tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 46kDaObserved band size: 46kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human small intestine using MBS1492185 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human small intestine using MBS1492185 at dilution 1:100)
Related Product Information for anti-FBXW4 antibody
Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Likely to be involved in key signaling pathways crucial for normal limb development. May participate in Wnt signaling.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,337 Da
NCBI Official Full Name
F-box/WD repeat-containing protein 4
NCBI Official Synonym Full Names
F-box and WD repeat domain containing 4
NCBI Official Symbol
FBXW4
NCBI Official Synonym Symbols
DAC; FBW4; FBWD4; SHFM3; SHSF3
NCBI Protein Information
F-box/WD repeat-containing protein 4
UniProt Protein Name
F-box/WD repeat-containing protein 4
UniProt Gene Name
FBXW4
UniProt Synonym Gene Names
FBW4; SHFM3
UniProt Entry Name
FBXW4_HUMAN

NCBI Description

This gene is a member of the F-box/WD-40 gene family, which recruit specific target proteins through their WD-40 protein-protein binding domains for ubiquitin mediated degradation. In mouse, a highly similar protein is thought to be responsible for maintaining the apical ectodermal ridge of developing limb buds; disruption of the mouse gene results in the absence of central digits, underdeveloped or absent metacarpal/metatarsal bones and syndactyly. This phenotype is remarkably similar to split hand-split foot malformation in humans, a clinically heterogeneous condition with a variety of modes of transmission. An autosomal recessive form has been mapped to the chromosomal region where this gene is located, and complex rearrangements involving duplications of this gene and others have been associated with the condition. A pseudogene of this locus has been mapped to one of the introns of the BCR gene on chromosome 22. [provided by RefSeq, Jul 2008]

Uniprot Description

FBXW4: Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Likely to be involved in key signaling pathways crucial for normal limb development. May participate in Wnt signaling. Defects in FBXW4 are a cause of split-hand/foot malformation type 3 (SHFM3). SHFM3 is an autosomal dominant disorder characterized by hypoplasia/aplasia of the central digits with fusion of the remaining digits.

Protein type: Cell cycle regulation; Cell development/differentiation

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: ubiquitin ligase complex

Biological Process: cartilage development; embryonic digit morphogenesis; embryonic limb morphogenesis; positive regulation of mesenchymal cell proliferation; ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway

Disease: Split-hand/foot Malformation 3

Research Articles on FBXW4

Similar Products

Product Notes

The FBXW4 fbxw4 (Catalog #AAA1492185) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human FBXW4 polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FBXW4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the FBXW4 fbxw4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FBXW4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.