Western Blot (WB)
(Western Blot analysis of MBS625768 in mouse spleen lysate. SENP8 (arrow) was detected using MBS625768. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Human, Mouse SENP8 Polyclonal Antibody | anti-SENP8 antibody
SENP8, NT (Sentrin-specific Protease 8, Sentrin/SUMO-specific Protease SENP8, Protease, Cysteine 2, NEDD8-specific Protease 1, Deneddylase-1, DEN1, NEDP1, PRSC2, FKSG8) (FITC)
Gene Names
SENP8; DEN1; NEDP1; PRSC2
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
SENP8; Polyclonal Antibody; NT (Sentrin-specific Protease 8; Sentrin/SUMO-specific Protease SENP8; Protease; Cysteine 2; NEDD8-specific Protease 1; Deneddylase-1; DEN1; NEDP1; PRSC2; FKSG8) (FITC); anti-SENP8 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SENP8. Species Crossreactivity: mouse
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Sequence Length
212
Applicable Applications for anti-SENP8 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-1:500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 4-35 in the N-terminal region of human SENP8, UniProt Accession #Q96LD8; Q9D2Z4; NP_660205.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of MBS625768 in mouse spleen lysate. SENP8 (arrow) was detected using MBS625768. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western Blot analysis of MBS625768 in mouse spleen lysate. SENP8 (arrow) was detected using MBS625768. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western Blot analysis of SENP8 (arrow) using MBS625768. 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SENP8 gene.)
Western Blot (WB)
(Western Blot analysis of SENP8 (arrow) using MBS625768. 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SENP8 gene.)
Product Categories/Family for anti-SENP8 antibody
References
1. Wu, K., et al., J. Biol. Chem. 278(31):28882-28891 (2003). 2. Gan-Erdene, T., et al., J. Biol. Chem. 278(31):28892-28900 (2003). 3. Mendoza, H.M., et al., J. Biol. Chem. 278(28):25637-25643 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24.107
NCBI Official Full Name
sentrin-specific protease 8
NCBI Official Synonym Full Names
SUMO peptidase family member, NEDD8 specific
NCBI Official Symbol
SENP8
NCBI Official Synonym Symbols
DEN1; NEDP1; PRSC2
NCBI Protein Information
sentrin-specific protease 8
UniProt Protein Name
Sentrin-specific protease 8
Protein Family
UniProt Gene Name
SENP8
UniProt Synonym Gene Names
DEN1; NEDP1; PRSC2
UniProt Entry Name
SENP8_HUMAN
NCBI Description
This gene encodes a cysteine protease that is a member of the sentrin-specific protease family. The encoded protein is involved in processing and deconjugation of the ubiquitin-like protein termed, neural precursor cell expressed developmentally downregulated 8. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]
Uniprot Description
SENP8: Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53. Belongs to the peptidase C48 family.
Protein type: EC 3.4.22.68; Protease
Chromosomal Location of Human Ortholog: 15q23
Cellular Component: cytoplasm; nucleus
Molecular Function: NEDD8-specific protease activity
Biological Process: protein deneddylation; proteolysis
Research Articles on SENP8
Similar Products
Product Notes
The SENP8 senp8 (Catalog #AAA6498871) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SENP8, NT (Sentrin-specific Protease 8, Sentrin/SUMO-specific Protease SENP8, Protease, Cysteine 2, NEDD8-specific Protease 1, Deneddylase-1, DEN1, NEDP1, PRSC2, FKSG8) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SENP8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-1:500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SENP8 senp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SENP8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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