Rabbit anti-Human, Mouse SENP8 Polyclonal Antibody | anti-SENP8 antibody

SENP8, NT (Sentrin-specific Protease 8, Sentrin/SUMO-specific Protease SENP8, Protease, Cysteine 2, NEDD8-specific Protease 1, Deneddylase-1, DEN1, NEDP1, PRSC2, FKSG8) (AP)

Cat. Num. AAA6498868

• Gene Names: SENP8; DEN1; NEDP1; PRSC2
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: SENP8; Polyclonal Antibody; NT (Sentrin-specific Protease 8; Sentrin/SUMO-specific Protease SENP8; Protease; Cysteine 2; NEDD8-specific Protease 1; Deneddylase-1; DEN1; NEDP1; PRSC2; FKSG8) (AP); anti-SENP8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SENP8. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 212

• Applicable Applications for anti-SENP8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 4-35 in the N-terminal region of human SENP8, UniProt Accession #Q96LD8; Q9D2Z4; NP_660205.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS625768 in mouse spleen lysate. SENP8 (arrow) was detected using MBS625768. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB)

(Western Blot analysis of SENP8 (arrow) using MBS625768. 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SENP8 gene.)

Product Categories/Family for anti-SENP8 antibody

Antibodies; Ubiquitin

References

1. Wu, K., et al., J. Biol. Chem. 278(31):28882-28891 (2003). 2. Gan-Erdene, T., et al., J. Biol. Chem. 278(31):28892-28900 (2003). 3. Mendoza, H.M., et al., J. Biol. Chem. 278(28):25637-25643 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 262118306
• NCBI GeneID: 123228
• NCBI Accession #: NP_660205
• NCBI GenBank Nucleotide #: NP_660205.3
• UniProt Accession #: Q96LD8; Q9D2Z4
• Molecular Weight: 24.107
• NCBI Official Full Name: sentrin-specific protease 8
• NCBI Official Synonym Full Names: SUMO peptidase family member, NEDD8 specific
• NCBI Official Symbol: SENP8
• NCBI Official Synonym Symbols: DEN1; NEDP1; PRSC2
• NCBI Protein Information: sentrin-specific protease 8
• UniProt Protein Name: Sentrin-specific protease 8
• Protein Family: Sentrin-specific protease
• UniProt Gene Name: SENP8
• UniProt Synonym Gene Names: DEN1; NEDP1; PRSC2
• UniProt Entry Name: SENP8_HUMAN

NCBI Description

This gene encodes a cysteine protease that is a member of the sentrin-specific protease family. The encoded protein is involved in processing and deconjugation of the ubiquitin-like protein termed, neural precursor cell expressed developmentally downregulated 8. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]

Uniprot Description

SENP8: Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53. Belongs to the peptidase C48 family.

Protein type: EC 3.4.22.68; Protease

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: cytoplasm; nucleus

Molecular Function: NEDD8-specific protease activity

Biological Process: protein deneddylation; proteolysis