Western Blot (WB)
(Figure-1: Western blot analysis of Semenogelin II. Anti- Semenogelin II antibody (20-1085) was used with 1: 5000 dilution on HT-29 lysate.)
Rabbit Semenogelin II Polyclonal Antibody | anti-SEMG2 antibody
Polyclonal Antibody to Semenogelin II
Gene Names
SEMG2; SGII
Reactivity
Rat, Mouse, Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Synonyms
Semenogelin II; Polyclonal Antibody; Polyclonal Antibody to Semenogelin II; SEMG2; anti-SEMG2 antibody
Host
Rabbit
Reactivity
Rat, Mouse, Human
Clonality
Polyclonal
Isotype
IgG
Form/Format
Sera
Sequence Length
582
Applicable Applications for anti-SEMG2 antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB)
Application Notes
WB: 1: 1000-1: 5000
IHC (paraffin): 1: 1000-1: 5000
IHC (frozen): User should optimize
IP: 1: 50-1: 200
IHC (paraffin): 1: 1000-1: 5000
IHC (frozen): User should optimize
IP: 1: 50-1: 200
Antigen
Semenogelin II
Immunogen Information
Gel purified rat p42 seminal vesicle protein II from rodent seminal vesicle secretion fluid (corresponding to secreted Semenogelin II) was used as immunogen for this antibody
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Western Blot (WB)
(Figure-1: Western blot analysis of Semenogelin II. Anti- Semenogelin II antibody (20-1085) was used with 1: 5000 dilution on HT-29 lysate.)
Western Blot (WB)
(Figure-1: Western blot analysis of Semenogelin II. Anti- Semenogelin II antibody (20-1085) was used with 1: 5000 dilution on HT-29 lysate.)
Related Product Information for anti-SEMG2 antibody
Semenogelin I (SgI) and Semenogelin II (SgII) are the major seminal vesicle secreted proteins in human semen. SgI and SgII, in semen both originate from the glandular epithelium of the seminal vesicles which secrete them at high concentrations. Human Sg1 is a non-glycosylated protein of 439 amino acids with a molecular weight of ~50 kDa. A few percent of the worlds population also carry an allele that gives rise to a truncated SgI molecule with a molecular mass of 43 kDa. Human SgII is a 559 amino acid protein with a molecular weight of approx 63 kDa. SgII has a potential site for N-linked glycosylation and approximately half of the molecules in human semen are glycosylated yielding two SgII species with a different of 5 kDa. SgI has a single Cys residue and SgII has two Cys residues, and the molecules can exist as covalent homo- and heteromultimers. Whereas SgI/SgII was originally identified as the major components present in seminal vesicle secretion, they are now known to be expressed in a number of tissues. The list includes seminal vesicles, epididymis, vas deferens, prostate, skeletal muscle, kidney, colon, trachea, lung, breast and retina as well as in several malignant tissues and cell lines. The non-genital expression of SgI/SgII suggests that these proteins also have functions that are unrelated to sperm.
Product Categories/Family for anti-SEMG2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,444 Da
NCBI Official Full Name
semenogelin-2
NCBI Official Synonym Full Names
semenogelin 2
NCBI Official Symbol
SEMG2
NCBI Official Synonym Symbols
SGII
NCBI Protein Information
semenogelin-2
UniProt Protein Name
Semenogelin-2
Protein Family
UniProt Gene Name
SEMG2
UniProt Synonym Gene Names
SGII
NCBI Description
The secreted protein encoded by this gene is involved in the formation of a gel matrix that encases ejaculated spermatozoa. Proteolysis by the prostate-specific antigen (PSA) breaks down the gel matrix and allows the spermatozoa to move more freely. The encoded protein is found in lesser abundance than a similar semenogelin protein. An antibacterial activity has been found for a antimicrobial peptide isolated from this protein. The genes encoding these two semenogelin proteins are found in a cluster on chromosome 20. [provided by RefSeq, Jan 2015]
Uniprot Description
Participates in the formation of a gel matrix (sperm coagulum) entrapping the accessory gland secretions and ejaculated spermatozoa.
Research Articles on SEMG2
Similar Products
Product Notes
The SEMG2 semg2 (Catalog #AAA669128) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Semenogelin II reacts with Rat, Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Semenogelin II can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB). WB: 1: 1000-1: 5000 IHC (paraffin): 1: 1000-1: 5000 IHC (frozen): User should optimize IP: 1: 50-1: 200. Researchers should empirically determine the suitability of the SEMG2 semg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Semenogelin II, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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