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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human Semaphorin 4D Polyclonal Antibody | anti-SEMA4D antibody

Polyclonal Antibody to Semaphorin 4D (SEMA4D)

Gene Names
SEMA4D; CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Semaphorin 4D; Polyclonal Antibody; Polyclonal Antibody to Semaphorin 4D (SEMA4D); anti-SEMA4D antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SEMA4D. It has beenselected for its ability to recognize SEMA4D in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-SEMA4D antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
SEMA4D (Phe502~Phe636)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human
Conjugate
No Conjugate
Immunogen
Recombinant SEMA4D (Phe502~Phe636) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2055077
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:HumanTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:HumanTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,164 Da
NCBI Official Full Name
semaphorin-4D isoform 2
NCBI Official Synonym Full Names
semaphorin 4D
NCBI Official Symbol
SEMA4D
NCBI Official Synonym Symbols
CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G
NCBI Protein Information
semaphorin-4D
UniProt Protein Name
Semaphorin-4D
Protein Family
UniProt Gene Name
SEMA4D
UniProt Synonym Gene Names
C9orf164; CD100; SEMAJ

Uniprot Description

SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: extracellular space; integral component of plasma membrane; plasma membrane

Molecular Function: protein binding; receptor activity; receptor binding; semaphorin receptor binding; transmembrane receptor activity

Biological Process: cell adhesion; immune response; negative chemotaxis; negative regulation of apoptosis; negative regulation of cell adhesion; negative regulation of osteoblast differentiation; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell migration; positive regulation of collateral sprouting; positive regulation of GTPase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein phosphorylation; regulation of cell projection organization; regulation of cell shape; regulation of dendrite morphogenesis

Research Articles on SEMA4D

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Product Notes

The SEMA4D sema4d (Catalog #AAA2027737) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Semaphorin 4D (SEMA4D) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Semaphorin 4D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the SEMA4D sema4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Semaphorin 4D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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